PRIMER_MIN_THREE_PRIME_DISTANCE=0
PRIMER_NUM_RETURN=2
PRIMER_OPT_SIZE=20
PRIMER_MAX_POLY_X=100
PRIMER_INTERNAL_OLIGO_MAX_POLY_X=100
PRIMER_PRODUCT_SIZE_RANGE=125-175 75-1000
PRIMER_MAX_GC=80.0
PRIMER_MAX_SIZE=25
PRIMER_MAX_TM=63.0
PRIMER_MIN_SIZE=18
PRIMER_MIN_GC=20
PRIMER_MIN_TM=57.0
PRIMER_OPT_TM=60.0
PRIMER_MAX_SELF_ANY=12
PRIMER_MAX_SELF_END=6
PRIMER_PAIR_MAX_COMPL_ANY=12
PRIMER_PAIR_MAX_COMPL_END=6
MARKER_NAME=01.RHAP33FA1.seq
SEQUENCE=NNNNNNNNNNNNNNNNNNNNGCCTTCATTAGAAAGTGAAAGTAAATGTTATCCTTTTAGATATGAATTAGCAAGTCATTATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGGAAACAGTATAAATAAGCAAAGATATTTGAATCCTGTCACCTTTTCATCCTCTGTCCTTTTGCCCAAGATAACATTGTTATAGTTGCCTTTACCCTGCACTGTACAACTCCTACTAAACTTCTTAGGTAGGAGGTGTGTTGGCTTTTCCTTGNGGGNTGGGTAGTCACAAAGTGTGTCTGAGCAAAGTCTCTTGCAAATCCCCTGGGTACTAANTAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
INCLUDED_REGION=20,315
TARGET=81,30,CA
PRIMER_PAIR_PENALTY=7.7339
PRIMER_LEFT_PENALTY=7.538496
PRIMER_RIGHT_PENALTY=0.195358
PRIMER_LEFT_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_SEQUENCE=GGCAAAAGGACAGAGGATGA
PRIMER_LEFT=20,25
PRIMER_RIGHT=177,20
PRIMER_LEFT_TM=57.462
PRIMER_RIGHT_TM=60.195
PRIMER_LEFT_GC_PERCENT=32.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=2.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=6.0000
PRIMER_RIGHT_END_STABILITY=6.6000
PRIMER_PAIR_MAX_COMPL_ANY=4.00
PRIMER_PAIR_MAX_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=158
PRIMER_PAIR_PENALTY_1=7.8680
PRIMER_LEFT_1_PENALTY=7.538496
PRIMER_RIGHT_1_PENALTY=0.329550
PRIMER_LEFT_1_SEQUENCE=GCCTTCATTAGAAAGTGAAAGTAAA
PRIMER_RIGHT_1_SEQUENCE=ATCTTGGGCAAAAGGACAGA
PRIMER_LEFT_1=20,25
PRIMER_RIGHT_1=183,20
PRIMER_LEFT_1_TM=57.462
PRIMER_RIGHT_1_TM=59.670
PRIMER_LEFT_1_GC_PERCENT=32.000
PRIMER_RIGHT_1_GC_PERCENT=45.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.0000
PRIMER_RIGHT_1_END_STABILITY=6.4000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=164
=
MARKER_NAME=01.RHAP34FA1.seq
SEQUENCE=NNNNNNNNNNNNNNNNNNNACTTGTCATTTCTCAGAGACATCTATACCTCAGAGACAAACTTCTCAGAAAACAACCCTAAGGTTGAACAAAACATGGCTGTATGTATCTATATTATTACACGCGCGTGGGNNANNNNNNNNNNNNNNNNCGTTGATTTTTTATACTAACAAGANNATGTCCATATAACATGCTAGTATATCATACACACATACNNNNNNNNNNNNNNNNTTATACNNNNNNNNNNNNNNNNAGGAATGTGTGTGCNACCTNATGNTTAACNNCAAGTGTACCCNNGAATNTGTNGNTGNTGGCNT
INCLUDED_REGION=19,296
TARGET=134,15,CA
TARGET=213,16,AT
TARGET=235,16,AT
=
PRIMER_NUM_RETURN=1
MARKER_NAME=03.RHAP34FA3.seq
SEQUENCE=TNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGCAGGAGGATGAAATGCAATCTGAAGAGAGCCCCTGAGCCAGTCCAGCCTCCCCATCATGGCCTTTGCCTTCCAACCCCTGCGTTCTTCCGGGCCTCTGTGAAATGCTATCTTGATAATGTCTGTAATCACTTGATTGCAGCCAAGCTAAAGGCTGGCCCTGACTGGGATGCAGAATGAAGAGTTGTGGGGAGGCGAGGGATAGCAGGAACGAACCCAAAAAACACACTGCCCTTTCACACAGTCAT
INCLUDED_REGION=0,278
TARGET=1,28,CA
=
MARKER_NAME=03.RHAP34FE3.seq
SEQUENCE=NNNNNNNNNNNNNNNNNTCATACAAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGCATATTATGTATATGCTGCATCTAACTTTATCCTTTCAGACAGCCTAGCTGCAGCTCAGGATGCTGTCTATTCCAGACTTACCTCAAACATGCAGCAGCAGCAGTACATGAAGAAATTGAATTATTCCATGTTAAGGCAAAGATAAACACTATCCACAAATATAGGCTTATTAGCNAGGTCTAAAGAAAAATTTCACTCACTAAGCACTCAA
INCLUDED_REGION=17,284
TARGET=27,60,AC
=
MARKER_NAME=04.RHAP33FE4.seq
SEQUENCE=NNNNNNNNNNNNNNNNNNTCCCAGAATGCAATGCCCAGAAAAGTCTCGCCCTCAATCTCATAGTTTCACTCAGCGTCTAGCACCGAGGAAAGTTTTGTGAGAAGGGAGGAGTAAGTACTGGAGAGAGAAGAGGAATTAAACAATGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGTGTGNNNNNNNNNNNNNNNNNNNNNNTGTGTGTGTGTATAGGTATACCGNATATGTGTGTGGTGTATAGGCATAGGTATATGTATATGTGCNNNNNNNNNNNNNGNNNNNNNNNNNNNNNCAGANAGAGAGACNANACCNCNCNNGGNGNNNCNNNNGNGCNNAGAGNGNGNCNGANNGNGAGNGACCAC
INCLUDED_REGION=18,367
TARGET=146,46,AT
TARGET=199,22,TA
TARGET=286,13,GT
TARGET=300,15,AG
PRIMER_PAIR_PENALTY=7.3535
PRIMER_LEFT_PENALTY=4.174047
PRIMER_RIGHT_PENALTY=3.179421
PRIMER_LEFT_SEQUENCE=TGAGAAGGGAGGAGTAAGTACTGG
PRIMER_RIGHT_SEQUENCE=CCTATGCCTATACACCACACACA
PRIMER_LEFT=97,24
PRIMER_RIGHT=270,23
PRIMER_LEFT_TM=60.174
PRIMER_RIGHT_TM=59.821
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=47.826
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=6.4000
PRIMER_PAIR_MAX_COMPL_ANY=4.00
PRIMER_PAIR_MAX_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=174
=
MARKER_NAME=05.RHAP33FA5.seq
SEQUENCE=NNNNNNNNNNCCTAATACTGTGAAGACAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGAGGTGTTCCAATAGAGAAGCAAGATCATGACACCCTCTGACCTTTGCCCCTGGCAACCATTTTGGGTATGTTCTCATGGCTCCCTCCACCCCCAGCCTCAGTTTCCCTGAGAGGGAAGGGGGATATAGGGAGAGGAGAGCCGNGCCCCGCCCCCATCCGGGATCANTGTTGGGGGAAGAATGNGTGCAAGCCAGGCCACCNGGGGTTAANGCGGTGACCTCAGCCGNGCGCCCCAGGAGGGGTNNNAGANCCTCNANTTCGTANTCNNTGAGATCAATAANAATGTTTCAATGTGTGAAA
INCLUDED_REGION=10,369
TARGET=30,44,GT
=
MARKER_NAME=05.RHAP33FE5.seq
SEQUENCE=NNNNNNNNNNNNNNCTTCAGTAANAGATTCTTATCACAGGAAAGAAGGTGGAGAATGATAAACAAGGAGTTCAACATCAACCTCGGGCCTGTATATGTATGAAGTACGTACACCCAAATCCTATACATACATGTTCGTGCAAACACTCAAATACTACATATACTTGTATATACAAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTATACAAACACTTATTTATTGGTTTTATTTTGCCTTAAAGGATTAAGTTGTGATTACTAATACATTTTATTTTGCTTTTGGAGACAGGGTCTTGCTAGGTATCCTAAGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
INCLUDED_REGION=14,306
TARGET=176,34,CA
PRIMER_PAIR_PENALTY=0.8585
PRIMER_LEFT_PENALTY=0.702456
PRIMER_RIGHT_PENALTY=0.156061
PRIMER_LEFT_SEQUENCE=CATGTTCGTGCAAACACTCA
PRIMER_RIGHT_SEQUENCE=AGACCCTGTCTCCAAAAGCA
PRIMER_LEFT=129,20
PRIMER_RIGHT=302,20
PRIMER_LEFT_TM=59.298
PRIMER_RIGHT_TM=59.844
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=1.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.4000
PRIMER_RIGHT_END_STABILITY=8.5000
PRIMER_PAIR_MAX_COMPL_ANY=4.00
PRIMER_PAIR_MAX_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=174
=
PRIMER_NUM_RETURN=40
MARKER_NAME=05.RHAP34FA5.seq
SEQUENCE=NNNNNNNNNACCTAACTAAAACAGACCATAAACTGAGAACCTAGTATTCATGCAGCTGAGACTTTGAAGGCCACTCTCATTCAGCCAGGTGTGTGAGGANCGCGCGGCCGACATCTACTACTACTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNACAACNNNNNNNNNNNNNNNNCACAAAGNNNNNNNNNNNNNNGTAGGACAGACAGAGATTAGACAGAGTAGTAAGGGGAGAGANNAGGTTGGTTGTGGGAAGGACCTTATTACTTCACAA
INCLUDED_REGION=9,297
TARGET=125,36,AC
TARGET=162,24,GA
TARGET=191,16,AG
TARGET=214,14,GA
PRIMER_PAIR_PENALTY=9.8843
PRIMER_LEFT_PENALTY=1.973952
PRIMER_RIGHT_PENALTY=7.910390
PRIMER_LEFT_SEQUENCE=GCGGCCGACATCTACTACTA
PRIMER_RIGHT_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT=103,20
PRIMER_RIGHT=268,25
PRIMER_LEFT_TM=58.026
PRIMER_RIGHT_TM=57.090
PRIMER_LEFT_GC_PERCENT=55.000
PRIMER_RIGHT_GC_PERCENT=44.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=4.00
PRIMER_RIGHT_SELF_END=1.00
PRIMER_LEFT_END_STABILITY=4.7000
PRIMER_RIGHT_END_STABILITY=6.0000
PRIMER_PAIR_MAX_COMPL_ANY=4.00
PRIMER_PAIR_MAX_COMPL_END=1.00
PRIMER_PRODUCT_SIZE=166
PRIMER_PAIR_PENALTY_1=10.0123
PRIMER_LEFT_1_PENALTY=2.101866
PRIMER_RIGHT_1_PENALTY=7.910390
PRIMER_LEFT_1_SEQUENCE=GCGGCCGACATCTACTACTAC
PRIMER_RIGHT_1_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_1=103,21
PRIMER_RIGHT_1=268,25
PRIMER_LEFT_1_TM=58.898
PRIMER_RIGHT_1_TM=57.090
PRIMER_LEFT_1_GC_PERCENT=57.143
PRIMER_RIGHT_1_GC_PERCENT=44.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.1000
PRIMER_RIGHT_1_END_STABILITY=6.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=166
PRIMER_PAIR_PENALTY_2=10.0792
PRIMER_LEFT_2_PENALTY=2.168831
PRIMER_RIGHT_2_PENALTY=7.910390
PRIMER_LEFT_2_SEQUENCE=GCGGCCGACATCTACTACTACT
PRIMER_RIGHT_2_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_2=103,22
PRIMER_RIGHT_2=268,25
PRIMER_LEFT_2_TM=59.831
PRIMER_RIGHT_2_TM=57.090
PRIMER_LEFT_2_GC_PERCENT=54.545
PRIMER_RIGHT_2_GC_PERCENT=44.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=5.4000
PRIMER_RIGHT_2_END_STABILITY=6.0000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=10.5142
PRIMER_LEFT_3_PENALTY=2.603822
PRIMER_RIGHT_3_PENALTY=7.910390
PRIMER_LEFT_3_SEQUENCE=CGCGGCCGACATCTACTACT
PRIMER_RIGHT_3_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_3=102,20
PRIMER_RIGHT_3=268,25
PRIMER_LEFT_3_TM=62.604
PRIMER_RIGHT_3_TM=57.090
PRIMER_LEFT_3_GC_PERCENT=60.000
PRIMER_RIGHT_3_GC_PERCENT=44.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=5.4000
PRIMER_RIGHT_3_END_STABILITY=6.0000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_3=167
PRIMER_PAIR_PENALTY_4=10.6566
PRIMER_LEFT_4_PENALTY=2.746227
PRIMER_RIGHT_4_PENALTY=7.910390
PRIMER_LEFT_4_SEQUENCE=CGCGGCCGACATCTACTAC
PRIMER_RIGHT_4_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_4=102,19
PRIMER_RIGHT_4=268,25
PRIMER_LEFT_4_TM=61.746
PRIMER_RIGHT_4_TM=57.090
PRIMER_LEFT_4_GC_PERCENT=63.158
PRIMER_RIGHT_4_GC_PERCENT=44.000
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=5.1000
PRIMER_RIGHT_4_END_STABILITY=6.0000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=167
PRIMER_PAIR_PENALTY_5=10.8467
PRIMER_LEFT_5_PENALTY=2.936346
PRIMER_RIGHT_5_PENALTY=7.910390
PRIMER_LEFT_5_SEQUENCE=CGCGGCCGACATCTACTA
PRIMER_RIGHT_5_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_5=102,18
PRIMER_RIGHT_5=268,25
PRIMER_LEFT_5_TM=60.936
PRIMER_RIGHT_5_TM=57.090
PRIMER_LEFT_5_GC_PERCENT=61.111
PRIMER_RIGHT_5_GC_PERCENT=44.000
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=4.7000
PRIMER_RIGHT_5_END_STABILITY=6.0000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_5=167
PRIMER_PAIR_PENALTY_6=10.9749
PRIMER_LEFT_6_PENALTY=3.064519
PRIMER_RIGHT_6_PENALTY=7.910390
PRIMER_LEFT_6_SEQUENCE=GCGGCCGACATCTACTACT
PRIMER_RIGHT_6_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_6=103,19
PRIMER_RIGHT_6=268,25
PRIMER_LEFT_6_TM=57.935
PRIMER_RIGHT_6_TM=57.090
PRIMER_LEFT_6_GC_PERCENT=57.895
PRIMER_RIGHT_6_GC_PERCENT=44.000
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.4000
PRIMER_RIGHT_6_END_STABILITY=6.0000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=166
PRIMER_PAIR_PENALTY_7=11.4300
PRIMER_LEFT_7_PENALTY=3.519592
PRIMER_RIGHT_7_PENALTY=7.910390
PRIMER_LEFT_7_SEQUENCE=CGCGGCCGACATCTACTACTA
PRIMER_RIGHT_7_SEQUENCE=TCTCTCCCCTTACTACTCTGTCTAA
PRIMER_LEFT_7=102,21
PRIMER_RIGHT_7=268,25
PRIMER_LEFT_7_TM=62.520
PRIMER_RIGHT_7_TM=57.090
PRIMER_LEFT_7_GC_PERCENT=57.143
PRIMER_RIGHT_7_GC_PERCENT=44.000
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=4.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=4.7000
PRIMER_RIGHT_7_END_STABILITY=6.0000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_7=167
PRIMER_PAIR_PENALTY_8=0.3479
PRIMER_LEFT_8_PENALTY=0.003322
PRIMER_RIGHT_8_PENALTY=0.344549
PRIMER_LEFT_8_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_8_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_8=54,20
PRIMER_RIGHT_8=294,20
PRIMER_LEFT_8_TM=59.997
PRIMER_RIGHT_8_TM=60.345
PRIMER_LEFT_8_GC_PERCENT=55.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=4.00
PRIMER_RIGHT_8_SELF_ANY=7.00
PRIMER_LEFT_8_SELF_END=3.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.4000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=5.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=241
PRIMER_PAIR_PENALTY_9=0.3873
PRIMER_LEFT_9_PENALTY=0.042732
PRIMER_RIGHT_9_PENALTY=0.344549
PRIMER_LEFT_9_SEQUENCE=GAAGGCCACTCTCATTCAGC
PRIMER_RIGHT_9_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_9=65,20
PRIMER_RIGHT_9=294,20
PRIMER_LEFT_9_TM=59.957
PRIMER_RIGHT_9_TM=60.345
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=4.00
PRIMER_RIGHT_9_SELF_ANY=7.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_9=230
PRIMER_PAIR_PENALTY_10=0.4783
PRIMER_LEFT_10_PENALTY=0.133741
PRIMER_RIGHT_10_PENALTY=0.344549
PRIMER_LEFT_10_SEQUENCE=AGCTGAGACTTTGAAGGCCA
PRIMER_RIGHT_10_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_10=53,20
PRIMER_RIGHT_10=294,20
PRIMER_LEFT_10_TM=60.134
PRIMER_RIGHT_10_TM=60.345
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=7.00
PRIMER_LEFT_10_SELF_END=4.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=11.2000
PRIMER_RIGHT_10_END_STABILITY=8.2000
PRIMER_PAIR_10_COMPL_ANY=5.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=242
PRIMER_PAIR_PENALTY_11=0.4783
PRIMER_LEFT_11_PENALTY=0.133741
PRIMER_RIGHT_11_PENALTY=0.344549
PRIMER_LEFT_11_SEQUENCE=CAGCTGAGACTTTGAAGGCC
PRIMER_RIGHT_11_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_11=52,20
PRIMER_RIGHT_11=294,20
PRIMER_LEFT_11_TM=60.134
PRIMER_RIGHT_11_TM=60.345
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=50.000
PRIMER_LEFT_11_SELF_ANY=6.00
PRIMER_RIGHT_11_SELF_ANY=7.00
PRIMER_LEFT_11_SELF_END=4.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=10.9000
PRIMER_RIGHT_11_END_STABILITY=8.2000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=243
PRIMER_PAIR_PENALTY_12=0.6053
PRIMER_LEFT_12_PENALTY=0.260757
PRIMER_RIGHT_12_PENALTY=0.344549
PRIMER_LEFT_12_SEQUENCE=CCACTCTCATTCAGCCAGGT
PRIMER_RIGHT_12_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_12=70,20
PRIMER_RIGHT_12=294,20
PRIMER_LEFT_12_TM=60.261
PRIMER_RIGHT_12_TM=60.345
PRIMER_LEFT_12_GC_PERCENT=55.000
PRIMER_RIGHT_12_GC_PERCENT=50.000
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=7.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=8.2000
PRIMER_PAIR_12_COMPL_ANY=3.00
PRIMER_PAIR_12_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_12=225
PRIMER_PAIR_PENALTY_13=0.6297
PRIMER_LEFT_13_PENALTY=0.285121
PRIMER_RIGHT_13_PENALTY=0.344549
PRIMER_LEFT_13_SEQUENCE=GCAGCTGAGACTTTGAAGGC
PRIMER_RIGHT_13_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_13=51,20
PRIMER_RIGHT_13=294,20
PRIMER_LEFT_13_TM=60.285
PRIMER_RIGHT_13_TM=60.345
PRIMER_LEFT_13_GC_PERCENT=55.000
PRIMER_RIGHT_13_GC_PERCENT=50.000
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_LEFT_13_SELF_END=4.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=8.2000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=244
PRIMER_PAIR_PENALTY_14=0.6540
PRIMER_LEFT_14_PENALTY=0.309402
PRIMER_RIGHT_14_PENALTY=0.344549
PRIMER_LEFT_14_SEQUENCE=CATTCAGCCAGGTGTGTGAG
PRIMER_RIGHT_14_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_14=77,20
PRIMER_RIGHT_14=294,20
PRIMER_LEFT_14_TM=60.309
PRIMER_RIGHT_14_TM=60.345
PRIMER_LEFT_14_GC_PERCENT=55.000
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=2.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.4000
PRIMER_RIGHT_14_END_STABILITY=8.2000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_14=218
PRIMER_PAIR_PENALTY_15=0.6540
PRIMER_LEFT_15_PENALTY=0.309402
PRIMER_RIGHT_15_PENALTY=0.344549
PRIMER_LEFT_15_SEQUENCE=CTCATTCAGCCAGGTGTGTG
PRIMER_RIGHT_15_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_15=75,20
PRIMER_RIGHT_15=294,20
PRIMER_LEFT_15_TM=60.309
PRIMER_RIGHT_15_TM=60.345
PRIMER_LEFT_15_GC_PERCENT=55.000
PRIMER_RIGHT_15_GC_PERCENT=50.000
PRIMER_LEFT_15_SELF_ANY=3.00
PRIMER_RIGHT_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=3.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=8.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_15=220
PRIMER_PAIR_PENALTY_16=0.9299
PRIMER_LEFT_16_PENALTY=0.585389
PRIMER_RIGHT_16_PENALTY=0.344549
PRIMER_LEFT_16_SEQUENCE=CTCTCATTCAGCCAGGTGTG
PRIMER_RIGHT_16_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_16=73,20
PRIMER_RIGHT_16=294,20
PRIMER_LEFT_16_TM=59.415
PRIMER_RIGHT_16_TM=60.345
PRIMER_LEFT_16_GC_PERCENT=55.000
PRIMER_RIGHT_16_GC_PERCENT=50.000
PRIMER_LEFT_16_SELF_ANY=3.00
PRIMER_RIGHT_16_SELF_ANY=7.00
PRIMER_LEFT_16_SELF_END=1.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=8.2000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_16=222
PRIMER_PAIR_PENALTY_17=0.9299
PRIMER_LEFT_17_PENALTY=0.585389
PRIMER_RIGHT_17_PENALTY=0.344549
PRIMER_LEFT_17_SEQUENCE=CACTCTCATTCAGCCAGGTG
PRIMER_RIGHT_17_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_17=71,20
PRIMER_RIGHT_17=294,20
PRIMER_LEFT_17_TM=59.415
PRIMER_RIGHT_17_TM=60.345
PRIMER_LEFT_17_GC_PERCENT=55.000
PRIMER_RIGHT_17_GC_PERCENT=50.000
PRIMER_LEFT_17_SELF_ANY=3.00
PRIMER_RIGHT_17_SELF_ANY=7.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=7.9000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=224
PRIMER_PAIR_PENALTY_18=0.9758
PRIMER_LEFT_18_PENALTY=0.631211
PRIMER_RIGHT_18_PENALTY=0.344549
PRIMER_LEFT_18_SEQUENCE=TTGAAGGCCACTCTCATTCA
PRIMER_RIGHT_18_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_18=63,20
PRIMER_RIGHT_18=294,20
PRIMER_LEFT_18_TM=59.369
PRIMER_RIGHT_18_TM=60.345
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=50.000
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=7.00
PRIMER_LEFT_18_SELF_END=5.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.9000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=232
PRIMER_PAIR_PENALTY_19=1.0308
PRIMER_LEFT_19_PENALTY=0.686256
PRIMER_RIGHT_19_PENALTY=0.344549
PRIMER_LEFT_19_SEQUENCE=TGCAGCTGAGACTTTGAAGG
PRIMER_RIGHT_19_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_19=50,20
PRIMER_RIGHT_19=294,20
PRIMER_LEFT_19_TM=59.314
PRIMER_RIGHT_19_TM=60.345
PRIMER_LEFT_19_GC_PERCENT=50.000
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=6.00
PRIMER_RIGHT_19_SELF_ANY=7.00
PRIMER_LEFT_19_SELF_END=3.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=8.2000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_19=245
PRIMER_PAIR_PENALTY_20=1.0833
PRIMER_LEFT_20_PENALTY=0.738752
PRIMER_RIGHT_20_PENALTY=0.344549
PRIMER_LEFT_20_SEQUENCE=TCTCATTCAGCCAGGTGTGT
PRIMER_RIGHT_20_SEQUENCE=TAAGGTCCTTCCCACAACCA
PRIMER_LEFT_20=74,20
PRIMER_RIGHT_20=294,20
PRIMER_LEFT_20_TM=59.261
PRIMER_RIGHT_20_TM=60.345
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=50.000
PRIMER_LEFT_20_SELF_ANY=3.00
PRIMER_RIGHT_20_SELF_ANY=7.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=8.2000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_20=221
PRIMER_PAIR_PENALTY_21=1.1833
PRIMER_LEFT_21_PENALTY=0.003322
PRIMER_RIGHT_21_PENALTY=1.180017
PRIMER_LEFT_21_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_21_SEQUENCE=GTCCTTCCCACAACCAACCT
PRIMER_LEFT_21=54,20
PRIMER_RIGHT_21=290,20
PRIMER_LEFT_21_TM=59.997
PRIMER_RIGHT_21_TM=61.180
PRIMER_LEFT_21_GC_PERCENT=55.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=4.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=3.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.4000
PRIMER_RIGHT_21_END_STABILITY=7.9000
PRIMER_PAIR_21_COMPL_ANY=5.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_21=237
PRIMER_PAIR_PENALTY_22=1.1833
PRIMER_LEFT_22_PENALTY=0.003322
PRIMER_RIGHT_22_PENALTY=1.180017
PRIMER_LEFT_22_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_22_SEQUENCE=AGGTCCTTCCCACAACCAAC
PRIMER_LEFT_22=54,20
PRIMER_RIGHT_22=292,20
PRIMER_LEFT_22_TM=59.997
PRIMER_RIGHT_22_TM=61.180
PRIMER_LEFT_22_GC_PERCENT=55.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=4.00
PRIMER_RIGHT_22_SELF_ANY=5.00
PRIMER_LEFT_22_SELF_END=3.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.4000
PRIMER_RIGHT_22_END_STABILITY=8.2000
PRIMER_PAIR_22_COMPL_ANY=5.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_22=239
PRIMER_PAIR_PENALTY_23=1.2102
PRIMER_LEFT_23_PENALTY=0.003322
PRIMER_RIGHT_23_PENALTY=1.206859
PRIMER_LEFT_23_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_23_SEQUENCE=GTCCTTCCCACAACCAACC
PRIMER_LEFT_23=54,20
PRIMER_RIGHT_23=290,19
PRIMER_LEFT_23_TM=59.997
PRIMER_RIGHT_23_TM=60.207
PRIMER_LEFT_23_GC_PERCENT=55.000
PRIMER_RIGHT_23_GC_PERCENT=57.895
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=3.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=5.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_23=237
PRIMER_PAIR_PENALTY_24=1.2102
PRIMER_LEFT_24_PENALTY=0.003322
PRIMER_RIGHT_24_PENALTY=1.206859
PRIMER_LEFT_24_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_24_SEQUENCE=GGTCCTTCCCACAACCAAC
PRIMER_LEFT_24=54,20
PRIMER_RIGHT_24=291,19
PRIMER_LEFT_24_TM=59.997
PRIMER_RIGHT_24_TM=60.207
PRIMER_LEFT_24_GC_PERCENT=55.000
PRIMER_RIGHT_24_GC_PERCENT=57.895
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=3.00
PRIMER_LEFT_24_SELF_END=3.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.4000
PRIMER_RIGHT_24_END_STABILITY=8.2000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_24=238
PRIMER_PAIR_PENALTY_25=1.2227
PRIMER_LEFT_25_PENALTY=0.042732
PRIMER_RIGHT_25_PENALTY=1.180017
PRIMER_LEFT_25_SEQUENCE=GAAGGCCACTCTCATTCAGC
PRIMER_RIGHT_25_SEQUENCE=GTCCTTCCCACAACCAACCT
PRIMER_LEFT_25=65,20
PRIMER_RIGHT_25=290,20
PRIMER_LEFT_25_TM=59.957
PRIMER_RIGHT_25_TM=61.180
PRIMER_LEFT_25_GC_PERCENT=55.000
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=4.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=2.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=8.2000
PRIMER_RIGHT_25_END_STABILITY=7.9000
PRIMER_PAIR_25_COMPL_ANY=5.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_25=226
PRIMER_PAIR_PENALTY_26=1.2227
PRIMER_LEFT_26_PENALTY=0.042732
PRIMER_RIGHT_26_PENALTY=1.180017
PRIMER_LEFT_26_SEQUENCE=GAAGGCCACTCTCATTCAGC
PRIMER_RIGHT_26_SEQUENCE=AGGTCCTTCCCACAACCAAC
PRIMER_LEFT_26=65,20
PRIMER_RIGHT_26=292,20
PRIMER_LEFT_26_TM=59.957
PRIMER_RIGHT_26_TM=61.180
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=4.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=2.00
PRIMER_RIGHT_26_SELF_END=0.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=8.2000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_26=228
PRIMER_PAIR_PENALTY_27=1.2496
PRIMER_LEFT_27_PENALTY=0.042732
PRIMER_RIGHT_27_PENALTY=1.206859
PRIMER_LEFT_27_SEQUENCE=GAAGGCCACTCTCATTCAGC
PRIMER_RIGHT_27_SEQUENCE=GTCCTTCCCACAACCAACC
PRIMER_LEFT_27=65,20
PRIMER_RIGHT_27=290,19
PRIMER_LEFT_27_TM=59.957
PRIMER_RIGHT_27_TM=60.207
PRIMER_LEFT_27_GC_PERCENT=55.000
PRIMER_RIGHT_27_GC_PERCENT=57.895
PRIMER_LEFT_27_SELF_ANY=4.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=2.00
PRIMER_RIGHT_27_SELF_END=0.00
PRIMER_LEFT_27_END_STABILITY=8.2000
PRIMER_RIGHT_27_END_STABILITY=8.2000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_27=226
PRIMER_PAIR_PENALTY_28=1.2496
PRIMER_LEFT_28_PENALTY=0.042732
PRIMER_RIGHT_28_PENALTY=1.206859
PRIMER_LEFT_28_SEQUENCE=GAAGGCCACTCTCATTCAGC
PRIMER_RIGHT_28_SEQUENCE=GGTCCTTCCCACAACCAAC
PRIMER_LEFT_28=65,20
PRIMER_RIGHT_28=291,19
PRIMER_LEFT_28_TM=59.957
PRIMER_RIGHT_28_TM=60.207
PRIMER_LEFT_28_GC_PERCENT=55.000
PRIMER_RIGHT_28_GC_PERCENT=57.895
PRIMER_LEFT_28_SELF_ANY=4.00
PRIMER_RIGHT_28_SELF_ANY=3.00
PRIMER_LEFT_28_SELF_END=2.00
PRIMER_RIGHT_28_SELF_END=0.00
PRIMER_LEFT_28_END_STABILITY=8.2000
PRIMER_RIGHT_28_END_STABILITY=8.2000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_28=227
PRIMER_PAIR_PENALTY_29=1.3138
PRIMER_LEFT_29_PENALTY=0.133741
PRIMER_RIGHT_29_PENALTY=1.180017
PRIMER_LEFT_29_SEQUENCE=AGCTGAGACTTTGAAGGCCA
PRIMER_RIGHT_29_SEQUENCE=GTCCTTCCCACAACCAACCT
PRIMER_LEFT_29=53,20
PRIMER_RIGHT_29=290,20
PRIMER_LEFT_29_TM=60.134
PRIMER_RIGHT_29_TM=61.180
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=2.00
PRIMER_LEFT_29_SELF_END=4.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=11.2000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=5.00
PRIMER_PAIR_29_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_29=238
PRIMER_PAIR_PENALTY_30=1.3138
PRIMER_LEFT_30_PENALTY=0.133741
PRIMER_RIGHT_30_PENALTY=1.180017
PRIMER_LEFT_30_SEQUENCE=CAGCTGAGACTTTGAAGGCC
PRIMER_RIGHT_30_SEQUENCE=GTCCTTCCCACAACCAACCT
PRIMER_LEFT_30=52,20
PRIMER_RIGHT_30=290,20
PRIMER_LEFT_30_TM=60.134
PRIMER_RIGHT_30_TM=61.180
PRIMER_LEFT_30_GC_PERCENT=55.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=6.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=4.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=10.9000
PRIMER_RIGHT_30_END_STABILITY=7.9000
PRIMER_PAIR_30_COMPL_ANY=5.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_30=239
PRIMER_PAIR_PENALTY_31=1.3138
PRIMER_LEFT_31_PENALTY=0.133741
PRIMER_RIGHT_31_PENALTY=1.180017
PRIMER_LEFT_31_SEQUENCE=AGCTGAGACTTTGAAGGCCA
PRIMER_RIGHT_31_SEQUENCE=AGGTCCTTCCCACAACCAAC
PRIMER_LEFT_31=53,20
PRIMER_RIGHT_31=292,20
PRIMER_LEFT_31_TM=60.134
PRIMER_RIGHT_31_TM=61.180
PRIMER_LEFT_31_GC_PERCENT=50.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=4.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=11.2000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_31=240
PRIMER_PAIR_PENALTY_32=1.3138
PRIMER_LEFT_32_PENALTY=0.133741
PRIMER_RIGHT_32_PENALTY=1.180017
PRIMER_LEFT_32_SEQUENCE=CAGCTGAGACTTTGAAGGCC
PRIMER_RIGHT_32_SEQUENCE=AGGTCCTTCCCACAACCAAC
PRIMER_LEFT_32=52,20
PRIMER_RIGHT_32=292,20
PRIMER_LEFT_32_TM=60.134
PRIMER_RIGHT_32_TM=61.180
PRIMER_LEFT_32_GC_PERCENT=55.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=6.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=4.00
PRIMER_RIGHT_32_SELF_END=0.00
PRIMER_LEFT_32_END_STABILITY=10.9000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=5.00
PRIMER_PAIR_32_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_32=241
PRIMER_PAIR_PENALTY_33=1.3406
PRIMER_LEFT_33_PENALTY=0.133741
PRIMER_RIGHT_33_PENALTY=1.206859
PRIMER_LEFT_33_SEQUENCE=AGCTGAGACTTTGAAGGCCA
PRIMER_RIGHT_33_SEQUENCE=GTCCTTCCCACAACCAACC
PRIMER_LEFT_33=53,20
PRIMER_RIGHT_33=290,19
PRIMER_LEFT_33_TM=60.134
PRIMER_RIGHT_33_TM=60.207
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=57.895
PRIMER_LEFT_33_SELF_ANY=4.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=4.00
PRIMER_RIGHT_33_SELF_END=0.00
PRIMER_LEFT_33_END_STABILITY=11.2000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_33=238
PRIMER_PAIR_PENALTY_34=1.3406
PRIMER_LEFT_34_PENALTY=0.133741
PRIMER_RIGHT_34_PENALTY=1.206859
PRIMER_LEFT_34_SEQUENCE=CAGCTGAGACTTTGAAGGCC
PRIMER_RIGHT_34_SEQUENCE=GTCCTTCCCACAACCAACC
PRIMER_LEFT_34=52,20
PRIMER_RIGHT_34=290,19
PRIMER_LEFT_34_TM=60.134
PRIMER_RIGHT_34_TM=60.207
PRIMER_LEFT_34_GC_PERCENT=55.000
PRIMER_RIGHT_34_GC_PERCENT=57.895
PRIMER_LEFT_34_SELF_ANY=6.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=4.00
PRIMER_RIGHT_34_SELF_END=0.00
PRIMER_LEFT_34_END_STABILITY=10.9000
PRIMER_RIGHT_34_END_STABILITY=8.2000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_34=239
PRIMER_PAIR_PENALTY_35=1.3406
PRIMER_LEFT_35_PENALTY=0.133741
PRIMER_RIGHT_35_PENALTY=1.206859
PRIMER_LEFT_35_SEQUENCE=AGCTGAGACTTTGAAGGCCA
PRIMER_RIGHT_35_SEQUENCE=GGTCCTTCCCACAACCAAC
PRIMER_LEFT_35=53,20
PRIMER_RIGHT_35=291,19
PRIMER_LEFT_35_TM=60.134
PRIMER_RIGHT_35_TM=60.207
PRIMER_LEFT_35_GC_PERCENT=50.000
PRIMER_RIGHT_35_GC_PERCENT=57.895
PRIMER_LEFT_35_SELF_ANY=4.00
PRIMER_RIGHT_35_SELF_ANY=3.00
PRIMER_LEFT_35_SELF_END=4.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=11.2000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=5.00
PRIMER_PAIR_35_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_35=239
PRIMER_PAIR_PENALTY_36=1.3406
PRIMER_LEFT_36_PENALTY=0.133741
PRIMER_RIGHT_36_PENALTY=1.206859
PRIMER_LEFT_36_SEQUENCE=CAGCTGAGACTTTGAAGGCC
PRIMER_RIGHT_36_SEQUENCE=GGTCCTTCCCACAACCAAC
PRIMER_LEFT_36=52,20
PRIMER_RIGHT_36=291,19
PRIMER_LEFT_36_TM=60.134
PRIMER_RIGHT_36_TM=60.207
PRIMER_LEFT_36_GC_PERCENT=55.000
PRIMER_RIGHT_36_GC_PERCENT=57.895
PRIMER_LEFT_36_SELF_ANY=6.00
PRIMER_RIGHT_36_SELF_ANY=3.00
PRIMER_LEFT_36_SELF_END=4.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=10.9000
PRIMER_RIGHT_36_END_STABILITY=8.2000
PRIMER_PAIR_36_COMPL_ANY=5.00
PRIMER_PAIR_36_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_36=240
PRIMER_PAIR_PENALTY_37=1.3504
PRIMER_LEFT_37_PENALTY=0.003322
PRIMER_RIGHT_37_PENALTY=1.347100
PRIMER_LEFT_37_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_37_SEQUENCE=TCCTTCCCACAACCAACCT
PRIMER_LEFT_37=54,20
PRIMER_RIGHT_37=289,19
PRIMER_LEFT_37_TM=59.997
PRIMER_RIGHT_37_TM=60.347
PRIMER_LEFT_37_GC_PERCENT=55.000
PRIMER_RIGHT_37_GC_PERCENT=52.632
PRIMER_LEFT_37_SELF_ANY=4.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=3.00
PRIMER_RIGHT_37_SELF_END=0.00
PRIMER_LEFT_37_END_STABILITY=9.4000
PRIMER_RIGHT_37_END_STABILITY=7.9000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_37=236
PRIMER_PAIR_PENALTY_38=1.3504
PRIMER_LEFT_38_PENALTY=0.003322
PRIMER_RIGHT_38_PENALTY=1.347100
PRIMER_LEFT_38_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_38_SEQUENCE=AGGTCCTTCCCACAACCAA
PRIMER_LEFT_38=54,20
PRIMER_RIGHT_38=292,19
PRIMER_LEFT_38_TM=59.997
PRIMER_RIGHT_38_TM=60.347
PRIMER_LEFT_38_GC_PERCENT=55.000
PRIMER_RIGHT_38_GC_PERCENT=52.632
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=3.00
PRIMER_RIGHT_38_SELF_END=0.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=8.2000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_38=239
PRIMER_PAIR_PENALTY_39=1.3504
PRIMER_LEFT_39_PENALTY=0.003322
PRIMER_RIGHT_39_PENALTY=1.347100
PRIMER_LEFT_39_SEQUENCE=GCTGAGACTTTGAAGGCCAC
PRIMER_RIGHT_39_SEQUENCE=AAGGTCCTTCCCACAACCA
PRIMER_LEFT_39=54,20
PRIMER_RIGHT_39=293,19
PRIMER_LEFT_39_TM=59.997
PRIMER_RIGHT_39_TM=60.347
PRIMER_LEFT_39_GC_PERCENT=55.000
PRIMER_RIGHT_39_GC_PERCENT=52.632
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=7.00
PRIMER_LEFT_39_SELF_END=3.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_39=240
=
MARKER_NAME=06.RHAP34FE6.seq
SEQUENCE=NNNNNNNNCCAACCCAAAAATACAGACGGANACACACACAAACAGAGACACAGACACAGACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGAGACGGGTAACACAGGGNATCTTGACTGATATAGAGGTACATGTCCAAACAAGAATAGGGTAGCGGGAAAGCACAGAAGAGAAGAAAAGACAGAGTTTTGCAGACCTTGGATTTTACCCTTATGCCACCTCACCAAAGATGTGATTCCCTATATGGTCAAAGA
INCLUDED_REGION=8,287
TARGET=61,68,CA
PRIMER_PAIR_PENALTY=2.0965
PRIMER_LEFT_PENALTY=1.153194
PRIMER_RIGHT_PENALTY=0.943292
PRIMER_LEFT_SEQUENCE=CAACCCAAAAATACAGACGGA
PRIMER_RIGHT_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT=9,21
PRIMER_RIGHT=148,20
PRIMER_LEFT_TM=59.847
PRIMER_RIGHT_TM=60.943
PRIMER_LEFT_GC_PERCENT=42.857
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=2.00
PRIMER_RIGHT_SELF_ANY=3.00
PRIMER_LEFT_SELF_END=0.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.6000
PRIMER_RIGHT_END_STABILITY=7.0000
PRIMER_PAIR_MAX_COMPL_ANY=6.00
PRIMER_PAIR_MAX_COMPL_END=4.00
PRIMER_PRODUCT_SIZE=140
PRIMER_PAIR_PENALTY_1=2.6236
PRIMER_LEFT_1_PENALTY=1.153194
PRIMER_RIGHT_1_PENALTY=1.470379
PRIMER_LEFT_1_SEQUENCE=CAACCCAAAAATACAGACGGA
PRIMER_RIGHT_1_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_1=9,21
PRIMER_RIGHT_1=148,19
PRIMER_LEFT_1_TM=59.847
PRIMER_RIGHT_1_TM=59.530
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=57.895
PRIMER_LEFT_1_SELF_ANY=2.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=0.00
PRIMER_RIGHT_1_SELF_END=2.00
PRIMER_LEFT_1_END_STABILITY=9.6000
PRIMER_RIGHT_1_END_STABILITY=6.7000
PRIMER_PAIR_1_COMPL_ANY=6.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=140
PRIMER_PAIR_PENALTY_2=2.9431
PRIMER_LEFT_2_PENALTY=1.999797
PRIMER_RIGHT_2_PENALTY=0.943292
PRIMER_LEFT_2_SEQUENCE=CCAACCCAAAAATACAGACG
PRIMER_RIGHT_2_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_2=8,20
PRIMER_RIGHT_2=148,20
PRIMER_LEFT_2_TM=58.000
PRIMER_RIGHT_2_TM=60.943
PRIMER_LEFT_2_GC_PERCENT=45.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=2.00
PRIMER_RIGHT_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=8.1000
PRIMER_RIGHT_2_END_STABILITY=7.0000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_2=141
PRIMER_PAIR_PENALTY_3=2.9431
PRIMER_LEFT_3_PENALTY=1.999797
PRIMER_RIGHT_3_PENALTY=0.943292
PRIMER_LEFT_3_SEQUENCE=CAACCCAAAAATACAGACGG
PRIMER_RIGHT_3_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_3=9,20
PRIMER_RIGHT_3=148,20
PRIMER_LEFT_3_TM=58.000
PRIMER_RIGHT_3_TM=60.943
PRIMER_LEFT_3_GC_PERCENT=45.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=9.6000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=6.00
PRIMER_PAIR_3_COMPL_END=5.00
PRIMER_PRODUCT_SIZE_3=140
PRIMER_PAIR_PENALTY_4=3.4069
PRIMER_LEFT_4_PENALTY=2.463612
PRIMER_RIGHT_4_PENALTY=0.943292
PRIMER_LEFT_4_SEQUENCE=CCAACCCAAAAATACAGACGG
PRIMER_RIGHT_4_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_4=8,21
PRIMER_RIGHT_4=148,20
PRIMER_LEFT_4_TM=61.464
PRIMER_RIGHT_4_TM=60.943
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=2.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.6000
PRIMER_RIGHT_4_END_STABILITY=7.0000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=5.00
PRIMER_PRODUCT_SIZE_4=141
PRIMER_PAIR_PENALTY_5=3.4702
PRIMER_LEFT_5_PENALTY=1.999797
PRIMER_RIGHT_5_PENALTY=1.470379
PRIMER_LEFT_5_SEQUENCE=CCAACCCAAAAATACAGACG
PRIMER_RIGHT_5_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_5=8,20
PRIMER_RIGHT_5=148,19
PRIMER_LEFT_5_TM=58.000
PRIMER_RIGHT_5_TM=59.530
PRIMER_LEFT_5_GC_PERCENT=45.000
PRIMER_RIGHT_5_GC_PERCENT=57.895
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=2.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_END_STABILITY=8.1000
PRIMER_RIGHT_5_END_STABILITY=6.7000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=141
PRIMER_PAIR_PENALTY_6=3.4702
PRIMER_LEFT_6_PENALTY=1.999797
PRIMER_RIGHT_6_PENALTY=1.470379
PRIMER_LEFT_6_SEQUENCE=CAACCCAAAAATACAGACGG
PRIMER_RIGHT_6_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_6=9,20
PRIMER_RIGHT_6=148,19
PRIMER_LEFT_6_TM=58.000
PRIMER_RIGHT_6_TM=59.530
PRIMER_LEFT_6_GC_PERCENT=45.000
PRIMER_RIGHT_6_GC_PERCENT=57.895
PRIMER_LEFT_6_SELF_ANY=2.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=1.00
PRIMER_RIGHT_6_SELF_END=2.00
PRIMER_LEFT_6_END_STABILITY=9.6000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=6.00
PRIMER_PAIR_6_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_6=140
PRIMER_PAIR_PENALTY_7=3.8768
PRIMER_LEFT_7_PENALTY=2.933539
PRIMER_RIGHT_7_PENALTY=0.943292
PRIMER_LEFT_7_SEQUENCE=AACCCAAAAATACAGACGGA
PRIMER_RIGHT_7_SEQUENCE=CCCTGTGTTACCCGTCTCAA
PRIMER_LEFT_7=10,20
PRIMER_RIGHT_7=148,20
PRIMER_LEFT_7_TM=57.066
PRIMER_RIGHT_7_TM=60.943
PRIMER_LEFT_7_GC_PERCENT=40.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=2.00
PRIMER_RIGHT_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=0.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.6000
PRIMER_RIGHT_7_END_STABILITY=7.0000
PRIMER_PAIR_7_COMPL_ANY=6.00
PRIMER_PAIR_7_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_7=139
PRIMER_PAIR_PENALTY_8=3.9340
PRIMER_LEFT_8_PENALTY=2.463612
PRIMER_RIGHT_8_PENALTY=1.470379
PRIMER_LEFT_8_SEQUENCE=CCAACCCAAAAATACAGACGG
PRIMER_RIGHT_8_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_8=8,21
PRIMER_RIGHT_8=148,19
PRIMER_LEFT_8_TM=61.464
PRIMER_RIGHT_8_TM=59.530
PRIMER_LEFT_8_GC_PERCENT=47.619
PRIMER_RIGHT_8_GC_PERCENT=57.895
PRIMER_LEFT_8_SELF_ANY=2.00
PRIMER_RIGHT_8_SELF_ANY=3.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=2.00
PRIMER_LEFT_8_END_STABILITY=9.6000
PRIMER_RIGHT_8_END_STABILITY=6.7000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_8=141
PRIMER_PAIR_PENALTY_9=4.4039
PRIMER_LEFT_9_PENALTY=2.933539
PRIMER_RIGHT_9_PENALTY=1.470379
PRIMER_LEFT_9_SEQUENCE=AACCCAAAAATACAGACGGA
PRIMER_RIGHT_9_SEQUENCE=CCCTGTGTTACCCGTCTCA
PRIMER_LEFT_9=10,20
PRIMER_RIGHT_9=148,19
PRIMER_LEFT_9_TM=57.066
PRIMER_RIGHT_9_TM=59.530
PRIMER_LEFT_9_GC_PERCENT=40.000
PRIMER_RIGHT_9_GC_PERCENT=57.895
PRIMER_LEFT_9_SELF_ANY=2.00
PRIMER_RIGHT_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=0.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_LEFT_9_END_STABILITY=9.6000
PRIMER_RIGHT_9_END_STABILITY=6.7000
PRIMER_PAIR_9_COMPL_ANY=6.00
PRIMER_PAIR_9_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_9=139
PRIMER_PAIR_PENALTY_10=4.4517
PRIMER_LEFT_10_PENALTY=4.436430
PRIMER_RIGHT_10_PENALTY=0.015253
PRIMER_LEFT_10_SEQUENCE=CACAAACAGAGACACAGACACAGA
PRIMER_RIGHT_10_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
PRIMER_LEFT_10=36,24
PRIMER_RIGHT_10=210,20
PRIMER_LEFT_10_TM=60.436
PRIMER_RIGHT_10_TM=60.015
PRIMER_LEFT_10_GC_PERCENT=45.833
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=1.00
PRIMER_RIGHT_10_SELF_ANY=2.00
PRIMER_LEFT_10_SELF_END=0.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.4000
PRIMER_RIGHT_10_END_STABILITY=6.9000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_10=175
PRIMER_PAIR_PENALTY_11=4.4977
PRIMER_LEFT_11_PENALTY=4.436430
PRIMER_RIGHT_11_PENALTY=0.061230
PRIMER_LEFT_11_SEQUENCE=CACAAACAGAGACACAGACACAGA
PRIMER_RIGHT_11_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_11=36,24
PRIMER_RIGHT_11=203,20
PRIMER_LEFT_11_TM=60.436
PRIMER_RIGHT_11_TM=60.061
PRIMER_LEFT_11_GC_PERCENT=45.833
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=1.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_LEFT_11_END_STABILITY=6.4000
PRIMER_RIGHT_11_END_STABILITY=5.9000
PRIMER_PAIR_11_COMPL_ANY=3.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=168
PRIMER_PAIR_PENALTY_12=4.5292
PRIMER_LEFT_12_PENALTY=4.513917
PRIMER_RIGHT_12_PENALTY=0.015253
PRIMER_LEFT_12_SEQUENCE=CACAAACAGAGACACAGACACAG
PRIMER_RIGHT_12_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
PRIMER_LEFT_12=36,23
PRIMER_RIGHT_12=210,20
PRIMER_LEFT_12_TM=58.486
PRIMER_RIGHT_12_TM=60.015
PRIMER_LEFT_12_GC_PERCENT=47.826
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=1.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_LEFT_12_SELF_END=1.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=6.9000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_12=175
PRIMER_PAIR_PENALTY_13=4.5751
PRIMER_LEFT_13_PENALTY=4.513917
PRIMER_RIGHT_13_PENALTY=0.061230
PRIMER_LEFT_13_SEQUENCE=CACAAACAGAGACACAGACACAG
PRIMER_RIGHT_13_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_13=36,23
PRIMER_RIGHT_13=203,20
PRIMER_LEFT_13_TM=58.486
PRIMER_RIGHT_13_TM=60.061
PRIMER_LEFT_13_GC_PERCENT=47.826
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=1.00
PRIMER_RIGHT_13_SELF_ANY=2.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=0.00
PRIMER_LEFT_13_END_STABILITY=6.7000
PRIMER_RIGHT_13_END_STABILITY=5.9000
PRIMER_PAIR_13_COMPL_ANY=3.00
PRIMER_PAIR_13_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_13=168
PRIMER_PAIR_PENALTY_14=4.6705
PRIMER_LEFT_14_PENALTY=4.609236
PRIMER_RIGHT_14_PENALTY=0.061230
PRIMER_LEFT_14_SEQUENCE=ACACAAACAGAGACACAGACACAG
PRIMER_RIGHT_14_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_14=35,24
PRIMER_RIGHT_14=203,20
PRIMER_LEFT_14_TM=59.391
PRIMER_RIGHT_14_TM=60.061
PRIMER_LEFT_14_GC_PERCENT=45.833
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=1.00
PRIMER_RIGHT_14_SELF_ANY=2.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=5.9000
PRIMER_PAIR_14_COMPL_ANY=3.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=169
PRIMER_PAIR_PENALTY_15=4.6843
PRIMER_LEFT_15_PENALTY=4.669014
PRIMER_RIGHT_15_PENALTY=0.015253
PRIMER_LEFT_15_SEQUENCE=CACAAACAGAGACACAGACACA
PRIMER_RIGHT_15_SEQUENCE=CTTCTGTGCTTTCCCGCTAC
PRIMER_LEFT_15=36,22
PRIMER_RIGHT_15=210,20
PRIMER_LEFT_15_TM=57.331
PRIMER_RIGHT_15_TM=60.015
PRIMER_LEFT_15_GC_PERCENT=45.455
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=1.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=6.9000
PRIMER_PAIR_15_COMPL_ANY=6.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=175
PRIMER_PAIR_PENALTY_16=4.7302
PRIMER_LEFT_16_PENALTY=4.669014
PRIMER_RIGHT_16_PENALTY=0.061230
PRIMER_LEFT_16_SEQUENCE=CACAAACAGAGACACAGACACA
PRIMER_RIGHT_16_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_16=36,22
PRIMER_RIGHT_16=203,20
PRIMER_LEFT_16_TM=57.331
PRIMER_RIGHT_16_TM=60.061
PRIMER_LEFT_16_GC_PERCENT=45.455
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=1.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_LEFT_16_SELF_END=0.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=6.4000
PRIMER_RIGHT_16_END_STABILITY=5.9000
PRIMER_PAIR_16_COMPL_ANY=3.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=168
PRIMER_PAIR_PENALTY_17=4.7302
PRIMER_LEFT_17_PENALTY=4.669014
PRIMER_RIGHT_17_PENALTY=0.061230
PRIMER_LEFT_17_SEQUENCE=CACACAAACAGAGACACAGACA
PRIMER_RIGHT_17_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_17=34,22
PRIMER_RIGHT_17=203,20
PRIMER_LEFT_17_TM=57.331
PRIMER_RIGHT_17_TM=60.061
PRIMER_LEFT_17_GC_PERCENT=45.455
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=1.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=0.00
PRIMER_LEFT_17_END_STABILITY=6.4000
PRIMER_RIGHT_17_END_STABILITY=5.9000
PRIMER_PAIR_17_COMPL_ANY=3.00
PRIMER_PAIR_17_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_17=170
PRIMER_PAIR_PENALTY_18=4.7302
PRIMER_LEFT_18_PENALTY=4.669014
PRIMER_RIGHT_18_PENALTY=0.061230
PRIMER_LEFT_18_SEQUENCE=CACACACAAACAGAGACACAGA
PRIMER_RIGHT_18_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_18=32,22
PRIMER_RIGHT_18=203,20
PRIMER_LEFT_18_TM=57.331
PRIMER_RIGHT_18_TM=60.061
PRIMER_LEFT_18_GC_PERCENT=45.455
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=1.00
PRIMER_RIGHT_18_SELF_ANY=2.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=6.4000
PRIMER_RIGHT_18_END_STABILITY=5.9000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=172
PRIMER_PAIR_PENALTY_19=4.7308
PRIMER_LEFT_19_PENALTY=4.669525
PRIMER_RIGHT_19_PENALTY=0.061230
PRIMER_LEFT_19_SEQUENCE=CACACAAACAGAGACACAGACAC
PRIMER_RIGHT_19_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_19=34,23
PRIMER_RIGHT_19=203,20
PRIMER_LEFT_19_TM=58.330
PRIMER_RIGHT_19_TM=60.061
PRIMER_LEFT_19_GC_PERCENT=47.826
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=1.00
PRIMER_RIGHT_19_SELF_ANY=2.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=6.1000
PRIMER_RIGHT_19_END_STABILITY=5.9000
PRIMER_PAIR_19_COMPL_ANY=3.00
PRIMER_PAIR_19_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_19=170
PRIMER_PAIR_PENALTY_20=4.7308
PRIMER_LEFT_20_PENALTY=4.669525
PRIMER_RIGHT_20_PENALTY=0.061230
PRIMER_LEFT_20_SEQUENCE=ACACAAACAGAGACACAGACACA
PRIMER_RIGHT_20_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_20=35,23
PRIMER_RIGHT_20=203,20
PRIMER_LEFT_20_TM=58.330
PRIMER_RIGHT_20_TM=60.061
PRIMER_LEFT_20_GC_PERCENT=43.478
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=1.00
PRIMER_RIGHT_20_SELF_ANY=2.00
PRIMER_LEFT_20_SELF_END=0.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.4000
PRIMER_RIGHT_20_END_STABILITY=5.9000
PRIMER_PAIR_20_COMPL_ANY=3.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_20=169
PRIMER_PAIR_PENALTY_21=4.7308
PRIMER_LEFT_21_PENALTY=4.669525
PRIMER_RIGHT_21_PENALTY=0.061230
PRIMER_LEFT_21_SEQUENCE=ACACACAAACAGAGACACAGACA
PRIMER_RIGHT_21_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_21=33,23
PRIMER_RIGHT_21=203,20
PRIMER_LEFT_21_TM=58.330
PRIMER_RIGHT_21_TM=60.061
PRIMER_LEFT_21_GC_PERCENT=43.478
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=1.00
PRIMER_RIGHT_21_SELF_ANY=2.00
PRIMER_LEFT_21_SELF_END=0.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=6.4000
PRIMER_RIGHT_21_END_STABILITY=5.9000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_21=171
PRIMER_PAIR_PENALTY_22=4.7308
PRIMER_LEFT_22_PENALTY=4.669525
PRIMER_RIGHT_22_PENALTY=0.061230
PRIMER_LEFT_22_SEQUENCE=CACACACAAACAGAGACACAGAC
PRIMER_RIGHT_22_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_22=32,23
PRIMER_RIGHT_22=203,20
PRIMER_LEFT_22_TM=58.330
PRIMER_RIGHT_22_TM=60.061
PRIMER_LEFT_22_GC_PERCENT=47.826
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=1.00
PRIMER_RIGHT_22_SELF_ANY=2.00
PRIMER_LEFT_22_SELF_END=1.00
PRIMER_RIGHT_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=6.4000
PRIMER_RIGHT_22_END_STABILITY=5.9000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_22=172
PRIMER_PAIR_PENALTY_23=4.7308
PRIMER_LEFT_23_PENALTY=4.669525
PRIMER_RIGHT_23_PENALTY=0.061230
PRIMER_LEFT_23_SEQUENCE=ACACACACAAACAGAGACACAGA
PRIMER_RIGHT_23_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_23=31,23
PRIMER_RIGHT_23=203,20
PRIMER_LEFT_23_TM=58.330
PRIMER_RIGHT_23_TM=60.061
PRIMER_LEFT_23_GC_PERCENT=43.478
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=1.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=6.4000
PRIMER_RIGHT_23_END_STABILITY=5.9000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_23=173
PRIMER_PAIR_PENALTY_24=4.8123
PRIMER_LEFT_24_PENALTY=4.751110
PRIMER_RIGHT_24_PENALTY=0.061230
PRIMER_LEFT_24_SEQUENCE=ACACACAAACAGAGACACAGACAC
PRIMER_RIGHT_24_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_24=33,24
PRIMER_RIGHT_24=203,20
PRIMER_LEFT_24_TM=59.249
PRIMER_RIGHT_24_TM=60.061
PRIMER_LEFT_24_GC_PERCENT=45.833
PRIMER_RIGHT_24_GC_PERCENT=55.000
PRIMER_LEFT_24_SELF_ANY=1.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=0.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.1000
PRIMER_RIGHT_24_END_STABILITY=5.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_24=171
PRIMER_PAIR_PENALTY_25=4.8123
PRIMER_LEFT_25_PENALTY=4.751110
PRIMER_RIGHT_25_PENALTY=0.061230
PRIMER_LEFT_25_SEQUENCE=ACACACACAAACAGAGACACAGAC
PRIMER_RIGHT_25_SEQUENCE=GCTTTCCCGCTACCCTATTC
PRIMER_LEFT_25=31,24
PRIMER_RIGHT_25=203,20
PRIMER_LEFT_25_TM=59.249
PRIMER_RIGHT_25_TM=60.061
PRIMER_LEFT_25_GC_PERCENT=45.833
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=1.00
PRIMER_RIGHT_25_SELF_ANY=2.00
PRIMER_LEFT_25_SELF_END=1.00
PRIMER_RIGHT_25_SELF_END=0.00
PRIMER_LEFT_25_END_STABILITY=6.4000
PRIMER_RIGHT_25_END_STABILITY=5.9000
PRIMER_PAIR_25_COMPL_ANY=3.00
PRIMER_PAIR_25_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_25=173
PRIMER_PAIR_PENALTY_26=4.9077
PRIMER_LEFT_26_PENALTY=4.436430
PRIMER_RIGHT_26_PENALTY=0.471231
PRIMER_LEFT_26_SEQUENCE=CACAAACAGAGACACAGACACAGA
PRIMER_RIGHT_26_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_26=36,24
PRIMER_RIGHT_26=205,20
PRIMER_LEFT_26_TM=60.436
PRIMER_RIGHT_26_TM=60.471
PRIMER_LEFT_26_GC_PERCENT=45.833
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=1.00
PRIMER_RIGHT_26_SELF_ANY=2.00
PRIMER_LEFT_26_SELF_END=0.00
PRIMER_RIGHT_26_SELF_END=2.00
PRIMER_LEFT_26_END_STABILITY=6.4000
PRIMER_RIGHT_26_END_STABILITY=7.1000
PRIMER_PAIR_26_COMPL_ANY=3.00
PRIMER_PAIR_26_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_26=170
PRIMER_PAIR_PENALTY_27=4.9851
PRIMER_LEFT_27_PENALTY=4.513917
PRIMER_RIGHT_27_PENALTY=0.471231
PRIMER_LEFT_27_SEQUENCE=CACAAACAGAGACACAGACACAG
PRIMER_RIGHT_27_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_27=36,23
PRIMER_RIGHT_27=205,20
PRIMER_LEFT_27_TM=58.486
PRIMER_RIGHT_27_TM=60.471
PRIMER_LEFT_27_GC_PERCENT=47.826
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=1.00
PRIMER_RIGHT_27_SELF_ANY=2.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=2.00
PRIMER_LEFT_27_END_STABILITY=6.7000
PRIMER_RIGHT_27_END_STABILITY=7.1000
PRIMER_PAIR_27_COMPL_ANY=3.00
PRIMER_PAIR_27_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_27=170
PRIMER_PAIR_PENALTY_28=5.0805
PRIMER_LEFT_28_PENALTY=4.609236
PRIMER_RIGHT_28_PENALTY=0.471231
PRIMER_LEFT_28_SEQUENCE=ACACAAACAGAGACACAGACACAG
PRIMER_RIGHT_28_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_28=35,24
PRIMER_RIGHT_28=205,20
PRIMER_LEFT_28_TM=59.391
PRIMER_RIGHT_28_TM=60.471
PRIMER_LEFT_28_GC_PERCENT=45.833
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=1.00
PRIMER_RIGHT_28_SELF_ANY=2.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=2.00
PRIMER_LEFT_28_END_STABILITY=6.7000
PRIMER_RIGHT_28_END_STABILITY=7.1000
PRIMER_PAIR_28_COMPL_ANY=3.00
PRIMER_PAIR_28_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_28=171
PRIMER_PAIR_PENALTY_29=5.0958
PRIMER_LEFT_29_PENALTY=1.153194
PRIMER_RIGHT_29_PENALTY=3.942558
PRIMER_LEFT_29_SEQUENCE=CAACCCAAAAATACAGACGGA
PRIMER_RIGHT_29_SEQUENCE=CCTGTGTTACCCGTCTCAA
PRIMER_LEFT_29=9,21
PRIMER_RIGHT_29=147,19
PRIMER_LEFT_29_TM=59.847
PRIMER_RIGHT_29_TM=57.057
PRIMER_LEFT_29_GC_PERCENT=42.857
PRIMER_RIGHT_29_GC_PERCENT=52.632
PRIMER_LEFT_29_SELF_ANY=2.00
PRIMER_RIGHT_29_SELF_ANY=3.00
PRIMER_LEFT_29_SELF_END=0.00
PRIMER_RIGHT_29_SELF_END=0.00
PRIMER_LEFT_29_END_STABILITY=9.6000
PRIMER_RIGHT_29_END_STABILITY=7.0000
PRIMER_PAIR_29_COMPL_ANY=6.00
PRIMER_PAIR_29_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_29=139
PRIMER_PAIR_PENALTY_30=5.1402
PRIMER_LEFT_30_PENALTY=4.669014
PRIMER_RIGHT_30_PENALTY=0.471231
PRIMER_LEFT_30_SEQUENCE=CACAAACAGAGACACAGACACA
PRIMER_RIGHT_30_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_30=36,22
PRIMER_RIGHT_30=205,20
PRIMER_LEFT_30_TM=57.331
PRIMER_RIGHT_30_TM=60.471
PRIMER_LEFT_30_GC_PERCENT=45.455
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=1.00
PRIMER_RIGHT_30_SELF_ANY=2.00
PRIMER_LEFT_30_SELF_END=0.00
PRIMER_RIGHT_30_SELF_END=2.00
PRIMER_LEFT_30_END_STABILITY=6.4000
PRIMER_RIGHT_30_END_STABILITY=7.1000
PRIMER_PAIR_30_COMPL_ANY=3.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_30=170
PRIMER_PAIR_PENALTY_31=5.1402
PRIMER_LEFT_31_PENALTY=4.669014
PRIMER_RIGHT_31_PENALTY=0.471231
PRIMER_LEFT_31_SEQUENCE=CACACAAACAGAGACACAGACA
PRIMER_RIGHT_31_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_31=34,22
PRIMER_RIGHT_31=205,20
PRIMER_LEFT_31_TM=57.331
PRIMER_RIGHT_31_TM=60.471
PRIMER_LEFT_31_GC_PERCENT=45.455
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=1.00
PRIMER_RIGHT_31_SELF_ANY=2.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=2.00
PRIMER_LEFT_31_END_STABILITY=6.4000
PRIMER_RIGHT_31_END_STABILITY=7.1000
PRIMER_PAIR_31_COMPL_ANY=3.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_31=172
PRIMER_PAIR_PENALTY_32=5.1402
PRIMER_LEFT_32_PENALTY=4.669014
PRIMER_RIGHT_32_PENALTY=0.471231
PRIMER_LEFT_32_SEQUENCE=CACACACAAACAGAGACACAGA
PRIMER_RIGHT_32_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_32=32,22
PRIMER_RIGHT_32=205,20
PRIMER_LEFT_32_TM=57.331
PRIMER_RIGHT_32_TM=60.471
PRIMER_LEFT_32_GC_PERCENT=45.455
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=1.00
PRIMER_RIGHT_32_SELF_ANY=2.00
PRIMER_LEFT_32_SELF_END=0.00
PRIMER_RIGHT_32_SELF_END=2.00
PRIMER_LEFT_32_END_STABILITY=6.4000
PRIMER_RIGHT_32_END_STABILITY=7.1000
PRIMER_PAIR_32_COMPL_ANY=3.00
PRIMER_PAIR_32_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_32=174
PRIMER_PAIR_PENALTY_33=5.1408
PRIMER_LEFT_33_PENALTY=4.669525
PRIMER_RIGHT_33_PENALTY=0.471231
PRIMER_LEFT_33_SEQUENCE=CACACAAACAGAGACACAGACAC
PRIMER_RIGHT_33_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_33=34,23
PRIMER_RIGHT_33=205,20
PRIMER_LEFT_33_TM=58.330
PRIMER_RIGHT_33_TM=60.471
PRIMER_LEFT_33_GC_PERCENT=47.826
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=1.00
PRIMER_RIGHT_33_SELF_ANY=2.00
PRIMER_LEFT_33_SELF_END=0.00
PRIMER_RIGHT_33_SELF_END=2.00
PRIMER_LEFT_33_END_STABILITY=6.1000
PRIMER_RIGHT_33_END_STABILITY=7.1000
PRIMER_PAIR_33_COMPL_ANY=3.00
PRIMER_PAIR_33_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_33=172
PRIMER_PAIR_PENALTY_34=5.1408
PRIMER_LEFT_34_PENALTY=4.669525
PRIMER_RIGHT_34_PENALTY=0.471231
PRIMER_LEFT_34_SEQUENCE=ACACAAACAGAGACACAGACACA
PRIMER_RIGHT_34_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_34=35,23
PRIMER_RIGHT_34=205,20
PRIMER_LEFT_34_TM=58.330
PRIMER_RIGHT_34_TM=60.471
PRIMER_LEFT_34_GC_PERCENT=43.478
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=1.00
PRIMER_RIGHT_34_SELF_ANY=2.00
PRIMER_LEFT_34_SELF_END=0.00
PRIMER_RIGHT_34_SELF_END=2.00
PRIMER_LEFT_34_END_STABILITY=6.4000
PRIMER_RIGHT_34_END_STABILITY=7.1000
PRIMER_PAIR_34_COMPL_ANY=3.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_34=171
PRIMER_PAIR_PENALTY_35=5.1408
PRIMER_LEFT_35_PENALTY=4.669525
PRIMER_RIGHT_35_PENALTY=0.471231
PRIMER_LEFT_35_SEQUENCE=ACACACAAACAGAGACACAGACA
PRIMER_RIGHT_35_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_35=33,23
PRIMER_RIGHT_35=205,20
PRIMER_LEFT_35_TM=58.330
PRIMER_RIGHT_35_TM=60.471
PRIMER_LEFT_35_GC_PERCENT=43.478
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=1.00
PRIMER_RIGHT_35_SELF_ANY=2.00
PRIMER_LEFT_35_SELF_END=0.00
PRIMER_RIGHT_35_SELF_END=2.00
PRIMER_LEFT_35_END_STABILITY=6.4000
PRIMER_RIGHT_35_END_STABILITY=7.1000
PRIMER_PAIR_35_COMPL_ANY=3.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_35=173
PRIMER_PAIR_PENALTY_36=5.1408
PRIMER_LEFT_36_PENALTY=4.669525
PRIMER_RIGHT_36_PENALTY=0.471231
PRIMER_LEFT_36_SEQUENCE=CACACACAAACAGAGACACAGAC
PRIMER_RIGHT_36_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_36=32,23
PRIMER_RIGHT_36=205,20
PRIMER_LEFT_36_TM=58.330
PRIMER_RIGHT_36_TM=60.471
PRIMER_LEFT_36_GC_PERCENT=47.826
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=1.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=1.00
PRIMER_RIGHT_36_SELF_END=2.00
PRIMER_LEFT_36_END_STABILITY=6.4000
PRIMER_RIGHT_36_END_STABILITY=7.1000
PRIMER_PAIR_36_COMPL_ANY=3.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_36=174
PRIMER_PAIR_PENALTY_37=5.1408
PRIMER_LEFT_37_PENALTY=4.669525
PRIMER_RIGHT_37_PENALTY=0.471231
PRIMER_LEFT_37_SEQUENCE=ACACACACAAACAGAGACACAGA
PRIMER_RIGHT_37_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_37=31,23
PRIMER_RIGHT_37=205,20
PRIMER_LEFT_37_TM=58.330
PRIMER_RIGHT_37_TM=60.471
PRIMER_LEFT_37_GC_PERCENT=43.478
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=1.00
PRIMER_RIGHT_37_SELF_ANY=2.00
PRIMER_LEFT_37_SELF_END=0.00
PRIMER_RIGHT_37_SELF_END=2.00
PRIMER_LEFT_37_END_STABILITY=6.4000
PRIMER_RIGHT_37_END_STABILITY=7.1000
PRIMER_PAIR_37_COMPL_ANY=3.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_37=175
PRIMER_PAIR_PENALTY_38=5.2223
PRIMER_LEFT_38_PENALTY=4.751110
PRIMER_RIGHT_38_PENALTY=0.471231
PRIMER_LEFT_38_SEQUENCE=ACACACAAACAGAGACACAGACAC
PRIMER_RIGHT_38_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_38=33,24
PRIMER_RIGHT_38=205,20
PRIMER_LEFT_38_TM=59.249
PRIMER_RIGHT_38_TM=60.471
PRIMER_LEFT_38_GC_PERCENT=45.833
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=1.00
PRIMER_RIGHT_38_SELF_ANY=2.00
PRIMER_LEFT_38_SELF_END=0.00
PRIMER_RIGHT_38_SELF_END=2.00
PRIMER_LEFT_38_END_STABILITY=6.1000
PRIMER_RIGHT_38_END_STABILITY=7.1000
PRIMER_PAIR_38_COMPL_ANY=3.00
PRIMER_PAIR_38_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_38=173
PRIMER_PAIR_PENALTY_39=5.2223
PRIMER_LEFT_39_PENALTY=4.751110
PRIMER_RIGHT_39_PENALTY=0.471231
PRIMER_LEFT_39_SEQUENCE=ACACACACAAACAGAGACACAGAC
PRIMER_RIGHT_39_SEQUENCE=GTGCTTTCCCGCTACCCTAT
PRIMER_LEFT_39=31,24
PRIMER_RIGHT_39=205,20
PRIMER_LEFT_39_TM=59.249
PRIMER_RIGHT_39_TM=60.471
PRIMER_LEFT_39_GC_PERCENT=45.833
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=1.00
PRIMER_RIGHT_39_SELF_ANY=2.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=2.00
PRIMER_LEFT_39_END_STABILITY=6.4000
PRIMER_RIGHT_39_END_STABILITY=7.1000
PRIMER_PAIR_39_COMPL_ANY=3.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_39=175
=
MARKER_NAME=07.RHAP34FE7.seq
SEQUENCE=NNNNNNNNNNNNNNNNNNCCAGGACTGGAGTTCTCAACTGGAAGCAGGGCATAATAAATATACACAGACTACTCTTTGGGTACAAACTGACAGGCAATTTCAAGGCTTAAAGTTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCATGCACACCCCCCCTCTCCACCTGAACCTCTACATGCGTGCACATGTTCACACATGTTCTCTCATTTGCTCTCACACTTGCTCTCATTACTCGCTCTCTAGACCTT
INCLUDED_REGION=18,283
TARGET=115,29,CT
TARGET=145,20,NG
TARGET=166,24,CA
PRIMER_PAIR_PENALTY=0.4846
PRIMER_LEFT_PENALTY=0.151542
PRIMER_RIGHT_PENALTY=0.333053
PRIMER_LEFT_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT=76,20
PRIMER_RIGHT=238,20
PRIMER_LEFT_TM=60.152
PRIMER_RIGHT_TM=60.333
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=50.000
PRIMER_LEFT_SELF_ANY=5.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=3.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=9.7000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_MAX_COMPL_ANY=4.00
PRIMER_PAIR_MAX_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=163
PRIMER_PAIR_PENALTY_1=0.6812
PRIMER_LEFT_1_PENALTY=0.151542
PRIMER_RIGHT_1_PENALTY=0.529649
PRIMER_LEFT_1_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_1_SEQUENCE=GCACGCATGTAGAGGTTCAG
PRIMER_LEFT_1=76,20
PRIMER_RIGHT_1=235,20
PRIMER_LEFT_1_TM=60.152
PRIMER_RIGHT_1_TM=59.470
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=5.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=7.0000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=160
PRIMER_PAIR_PENALTY_2=0.7897
PRIMER_LEFT_2_PENALTY=0.151542
PRIMER_RIGHT_2_PENALTY=0.638191
PRIMER_LEFT_2_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_2_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_2=76,20
PRIMER_RIGHT_2=225,20
PRIMER_LEFT_2_TM=60.152
PRIMER_RIGHT_2_TM=60.638
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=60.000
PRIMER_LEFT_2_SELF_ANY=5.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_LEFT_2_SELF_END=3.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=9.7000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=150
PRIMER_PAIR_PENALTY_3=0.8285
PRIMER_LEFT_3_PENALTY=0.151542
PRIMER_RIGHT_3_PENALTY=0.676947
PRIMER_LEFT_3_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_3_SEQUENCE=GTGCACGCATGTAGAGGTTC
PRIMER_LEFT_3=76,20
PRIMER_RIGHT_3=237,20
PRIMER_LEFT_3_TM=60.152
PRIMER_RIGHT_3_TM=59.323
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=5.00
PRIMER_RIGHT_3_SELF_ANY=6.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=9.7000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=162
PRIMER_PAIR_PENALTY_4=0.8378
PRIMER_LEFT_4_PENALTY=0.151542
PRIMER_RIGHT_4_PENALTY=0.686256
PRIMER_LEFT_4_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_4_SEQUENCE=CACGCATGTAGAGGTTCAGG
PRIMER_LEFT_4=76,20
PRIMER_RIGHT_4=234,20
PRIMER_LEFT_4_TM=60.152
PRIMER_RIGHT_4_TM=59.314
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=9.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=159
PRIMER_PAIR_PENALTY_5=0.8378
PRIMER_LEFT_5_PENALTY=0.151542
PRIMER_RIGHT_5_PENALTY=0.686256
PRIMER_LEFT_5_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_5_SEQUENCE=CGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_5=76,20
PRIMER_RIGHT_5=232,20
PRIMER_LEFT_5_TM=60.152
PRIMER_RIGHT_5_TM=59.314
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=5.00
PRIMER_RIGHT_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=2.00
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=157
PRIMER_PAIR_PENALTY_6=0.9180
PRIMER_LEFT_6_PENALTY=0.151542
PRIMER_RIGHT_6_PENALTY=0.766499
PRIMER_LEFT_6_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_6_SEQUENCE=TGAACATGTGCACGCATGTA
PRIMER_LEFT_6=76,20
PRIMER_RIGHT_6=244,20
PRIMER_LEFT_6_TM=60.152
PRIMER_RIGHT_6_TM=60.766
PRIMER_LEFT_6_GC_PERCENT=50.000
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_LEFT_6_SELF_ANY=5.00
PRIMER_RIGHT_6_SELF_ANY=8.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=6.00
PRIMER_LEFT_6_END_STABILITY=9.7000
PRIMER_RIGHT_6_END_STABILITY=5.6000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=169
PRIMER_PAIR_PENALTY_7=0.9562
PRIMER_LEFT_7_PENALTY=0.151542
PRIMER_RIGHT_7_PENALTY=0.804687
PRIMER_LEFT_7_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_7_SEQUENCE=ATGTGCACGCATGTAGAGGT
PRIMER_LEFT_7=76,20
PRIMER_RIGHT_7=239,20
PRIMER_LEFT_7_TM=60.152
PRIMER_RIGHT_7_TM=59.195
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=5.00
PRIMER_RIGHT_7_SELF_ANY=6.00
PRIMER_LEFT_7_SELF_END=3.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=4.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_7=164
PRIMER_PAIR_PENALTY_8=1.0167
PRIMER_LEFT_8_PENALTY=0.683672
PRIMER_RIGHT_8_PENALTY=0.333053
PRIMER_LEFT_8_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_8_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_8=88,20
PRIMER_RIGHT_8=238,20
PRIMER_LEFT_8_TM=59.316
PRIMER_RIGHT_8_TM=60.333
PRIMER_LEFT_8_GC_PERCENT=45.000
PRIMER_RIGHT_8_GC_PERCENT=50.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=5.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=9.7000
PRIMER_RIGHT_8_END_STABILITY=7.9000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_8=151
PRIMER_PAIR_PENALTY_9=1.1088
PRIMER_LEFT_9_PENALTY=0.775718
PRIMER_RIGHT_9_PENALTY=0.333053
PRIMER_LEFT_9_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_9_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_9=86,20
PRIMER_RIGHT_9=238,20
PRIMER_LEFT_9_TM=60.776
PRIMER_RIGHT_9_TM=60.333
PRIMER_LEFT_9_GC_PERCENT=50.000
PRIMER_RIGHT_9_GC_PERCENT=50.000
PRIMER_LEFT_9_SELF_ANY=5.00
PRIMER_RIGHT_9_SELF_ANY=6.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.7000
PRIMER_RIGHT_9_END_STABILITY=7.9000
PRIMER_PAIR_9_COMPL_ANY=4.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=153
PRIMER_PAIR_PENALTY_10=1.1088
PRIMER_LEFT_10_PENALTY=0.775718
PRIMER_RIGHT_10_PENALTY=0.333053
PRIMER_LEFT_10_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_10_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_10=87,20
PRIMER_RIGHT_10=238,20
PRIMER_LEFT_10_TM=60.776
PRIMER_RIGHT_10_TM=60.333
PRIMER_LEFT_10_GC_PERCENT=45.000
PRIMER_RIGHT_10_GC_PERCENT=50.000
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=9.4000
PRIMER_RIGHT_10_END_STABILITY=7.9000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_10=152
PRIMER_PAIR_PENALTY_11=1.2133
PRIMER_LEFT_11_PENALTY=0.683672
PRIMER_RIGHT_11_PENALTY=0.529649
PRIMER_LEFT_11_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_11_SEQUENCE=GCACGCATGTAGAGGTTCAG
PRIMER_LEFT_11=88,20
PRIMER_RIGHT_11=235,20
PRIMER_LEFT_11_TM=59.316
PRIMER_RIGHT_11_TM=59.470
PRIMER_LEFT_11_GC_PERCENT=45.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=5.00
PRIMER_RIGHT_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=5.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=9.7000
PRIMER_RIGHT_11_END_STABILITY=7.0000
PRIMER_PAIR_11_COMPL_ANY=4.00
PRIMER_PAIR_11_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_11=148
PRIMER_PAIR_PENALTY_12=1.2778
PRIMER_LEFT_12_PENALTY=0.151542
PRIMER_RIGHT_12_PENALTY=1.126283
PRIMER_LEFT_12_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_12_SEQUENCE=GCATGTAGAGGTTCAGGTGGA
PRIMER_LEFT_12=76,20
PRIMER_RIGHT_12=231,21
PRIMER_LEFT_12_TM=60.152
PRIMER_RIGHT_12_TM=60.126
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=52.381
PRIMER_LEFT_12_SELF_ANY=5.00
PRIMER_RIGHT_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=3.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_END_STABILITY=9.7000
PRIMER_RIGHT_12_END_STABILITY=7.9000
PRIMER_PAIR_12_COMPL_ANY=4.00
PRIMER_PAIR_12_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_12=156
PRIMER_PAIR_PENALTY_13=1.3054
PRIMER_LEFT_13_PENALTY=0.775718
PRIMER_RIGHT_13_PENALTY=0.529649
PRIMER_LEFT_13_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_13_SEQUENCE=GCACGCATGTAGAGGTTCAG
PRIMER_LEFT_13=86,20
PRIMER_RIGHT_13=235,20
PRIMER_LEFT_13_TM=60.776
PRIMER_RIGHT_13_TM=59.470
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=5.00
PRIMER_RIGHT_13_SELF_ANY=4.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_LEFT_13_END_STABILITY=9.7000
PRIMER_RIGHT_13_END_STABILITY=7.0000
PRIMER_PAIR_13_COMPL_ANY=4.00
PRIMER_PAIR_13_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_13=150
PRIMER_PAIR_PENALTY_14=1.3054
PRIMER_LEFT_14_PENALTY=0.775718
PRIMER_RIGHT_14_PENALTY=0.529649
PRIMER_LEFT_14_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_14_SEQUENCE=GCACGCATGTAGAGGTTCAG
PRIMER_LEFT_14=87,20
PRIMER_RIGHT_14=235,20
PRIMER_LEFT_14_TM=60.776
PRIMER_RIGHT_14_TM=59.470
PRIMER_LEFT_14_GC_PERCENT=45.000
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=4.00
PRIMER_LEFT_14_SELF_END=3.00
PRIMER_RIGHT_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=9.4000
PRIMER_RIGHT_14_END_STABILITY=7.0000
PRIMER_PAIR_14_COMPL_ANY=4.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=149
PRIMER_PAIR_PENALTY_15=1.3219
PRIMER_LEFT_15_PENALTY=0.683672
PRIMER_RIGHT_15_PENALTY=0.638191
PRIMER_LEFT_15_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_15_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_15=88,20
PRIMER_RIGHT_15=225,20
PRIMER_LEFT_15_TM=59.316
PRIMER_RIGHT_15_TM=60.638
PRIMER_LEFT_15_GC_PERCENT=45.000
PRIMER_RIGHT_15_GC_PERCENT=60.000
PRIMER_LEFT_15_SELF_ANY=5.00
PRIMER_RIGHT_15_SELF_ANY=2.00
PRIMER_LEFT_15_SELF_END=5.00
PRIMER_RIGHT_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=9.7000
PRIMER_RIGHT_15_END_STABILITY=9.4000
PRIMER_PAIR_15_COMPL_ANY=2.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_15=138
PRIMER_PAIR_PENALTY_16=1.3375
PRIMER_LEFT_16_PENALTY=0.151542
PRIMER_RIGHT_16_PENALTY=1.185942
PRIMER_LEFT_16_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_16_SEQUENCE=CACGCATGTAGAGGTTCAGGT
PRIMER_LEFT_16=76,20
PRIMER_RIGHT_16=234,21
PRIMER_LEFT_16_TM=60.152
PRIMER_RIGHT_16_TM=60.186
PRIMER_LEFT_16_GC_PERCENT=50.000
PRIMER_RIGHT_16_GC_PERCENT=52.381
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_LEFT_16_END_STABILITY=9.7000
PRIMER_RIGHT_16_END_STABILITY=7.9000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_16=159
PRIMER_PAIR_PENALTY_17=1.3375
PRIMER_LEFT_17_PENALTY=0.151542
PRIMER_RIGHT_17_PENALTY=1.185942
PRIMER_LEFT_17_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_17_SEQUENCE=ACGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_17=76,20
PRIMER_RIGHT_17=233,21
PRIMER_LEFT_17_TM=60.152
PRIMER_RIGHT_17_TM=60.186
PRIMER_LEFT_17_GC_PERCENT=50.000
PRIMER_RIGHT_17_GC_PERCENT=52.381
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_LEFT_17_END_STABILITY=9.7000
PRIMER_RIGHT_17_END_STABILITY=7.9000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_17=158
PRIMER_PAIR_PENALTY_18=1.3484
PRIMER_LEFT_18_PENALTY=0.151542
PRIMER_RIGHT_18_PENALTY=1.196903
PRIMER_LEFT_18_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_18_SEQUENCE=AACATGTGCACGCATGTAGAG
PRIMER_LEFT_18=76,20
PRIMER_RIGHT_18=242,21
PRIMER_LEFT_18_TM=60.152
PRIMER_RIGHT_18_TM=59.803
PRIMER_LEFT_18_GC_PERCENT=50.000
PRIMER_RIGHT_18_GC_PERCENT=47.619
PRIMER_LEFT_18_SELF_ANY=5.00
PRIMER_RIGHT_18_SELF_ANY=8.00
PRIMER_LEFT_18_SELF_END=3.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_LEFT_18_END_STABILITY=9.7000
PRIMER_RIGHT_18_END_STABILITY=5.7000
PRIMER_PAIR_18_COMPL_ANY=4.00
PRIMER_PAIR_18_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_18=167
PRIMER_PAIR_PENALTY_19=1.3558
PRIMER_LEFT_19_PENALTY=1.022788
PRIMER_RIGHT_19_PENALTY=0.333053
PRIMER_LEFT_19_SEQUENCE=TTTGGGTACAAACTGACAGGC
PRIMER_RIGHT_19_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_19=74,21
PRIMER_RIGHT_19=238,20
PRIMER_LEFT_19_TM=60.023
PRIMER_RIGHT_19_TM=60.333
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=50.000
PRIMER_LEFT_19_SELF_ANY=5.00
PRIMER_RIGHT_19_SELF_ANY=6.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_LEFT_19_END_STABILITY=9.7000
PRIMER_RIGHT_19_END_STABILITY=7.9000
PRIMER_PAIR_19_COMPL_ANY=4.00
PRIMER_PAIR_19_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_19=165
PRIMER_PAIR_PENALTY_20=1.3606
PRIMER_LEFT_20_PENALTY=0.683672
PRIMER_RIGHT_20_PENALTY=0.676947
PRIMER_LEFT_20_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_20_SEQUENCE=GTGCACGCATGTAGAGGTTC
PRIMER_LEFT_20=88,20
PRIMER_RIGHT_20=237,20
PRIMER_LEFT_20_TM=59.316
PRIMER_RIGHT_20_TM=59.323
PRIMER_LEFT_20_GC_PERCENT=45.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=5.00
PRIMER_RIGHT_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=9.7000
PRIMER_RIGHT_20_END_STABILITY=7.9000
PRIMER_PAIR_20_COMPL_ANY=4.00
PRIMER_PAIR_20_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_20=150
PRIMER_PAIR_PENALTY_21=1.3699
PRIMER_LEFT_21_PENALTY=0.683672
PRIMER_RIGHT_21_PENALTY=0.686256
PRIMER_LEFT_21_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_21_SEQUENCE=CACGCATGTAGAGGTTCAGG
PRIMER_LEFT_21=88,20
PRIMER_RIGHT_21=234,20
PRIMER_LEFT_21_TM=59.316
PRIMER_RIGHT_21_TM=59.314
PRIMER_LEFT_21_GC_PERCENT=45.000
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=5.00
PRIMER_RIGHT_21_SELF_ANY=4.00
PRIMER_LEFT_21_SELF_END=5.00
PRIMER_RIGHT_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=9.7000
PRIMER_RIGHT_21_END_STABILITY=8.2000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_21=147
PRIMER_PAIR_PENALTY_22=1.3699
PRIMER_LEFT_22_PENALTY=0.683672
PRIMER_RIGHT_22_PENALTY=0.686256
PRIMER_LEFT_22_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_22_SEQUENCE=CGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_22=88,20
PRIMER_RIGHT_22=232,20
PRIMER_LEFT_22_TM=59.316
PRIMER_RIGHT_22_TM=59.314
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_LEFT_22_SELF_END=5.00
PRIMER_RIGHT_22_SELF_END=2.00
PRIMER_LEFT_22_END_STABILITY=9.7000
PRIMER_RIGHT_22_END_STABILITY=7.9000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_22=145
PRIMER_PAIR_PENALTY_23=1.4139
PRIMER_LEFT_23_PENALTY=0.775718
PRIMER_RIGHT_23_PENALTY=0.638191
PRIMER_LEFT_23_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_23_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_23=87,20
PRIMER_RIGHT_23=225,20
PRIMER_LEFT_23_TM=60.776
PRIMER_RIGHT_23_TM=60.638
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=60.000
PRIMER_LEFT_23_SELF_ANY=4.00
PRIMER_RIGHT_23_SELF_ANY=2.00
PRIMER_LEFT_23_SELF_END=3.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_LEFT_23_END_STABILITY=9.4000
PRIMER_RIGHT_23_END_STABILITY=9.4000
PRIMER_PAIR_23_COMPL_ANY=3.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_23=139
PRIMER_PAIR_PENALTY_24=1.4139
PRIMER_LEFT_24_PENALTY=0.775718
PRIMER_RIGHT_24_PENALTY=0.638191
PRIMER_LEFT_24_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_24_SEQUENCE=AGAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_24=86,20
PRIMER_RIGHT_24=225,20
PRIMER_LEFT_24_TM=60.776
PRIMER_RIGHT_24_TM=60.638
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=60.000
PRIMER_LEFT_24_SELF_ANY=5.00
PRIMER_RIGHT_24_SELF_ANY=2.00
PRIMER_LEFT_24_SELF_END=2.00
PRIMER_RIGHT_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=9.7000
PRIMER_RIGHT_24_END_STABILITY=9.4000
PRIMER_PAIR_24_COMPL_ANY=4.00
PRIMER_PAIR_24_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_24=140
PRIMER_PAIR_PENALTY_25=1.4178
PRIMER_LEFT_25_PENALTY=0.151542
PRIMER_RIGHT_25_PENALTY=1.266285
PRIMER_LEFT_25_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_25_SEQUENCE=AACATGTGCACGCATGTAGA
PRIMER_LEFT_25=76,20
PRIMER_RIGHT_25=242,20
PRIMER_LEFT_25_TM=60.152
PRIMER_RIGHT_25_TM=58.734
PRIMER_LEFT_25_GC_PERCENT=50.000
PRIMER_RIGHT_25_GC_PERCENT=45.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=8.00
PRIMER_LEFT_25_SELF_END=3.00
PRIMER_RIGHT_25_SELF_END=4.00
PRIMER_LEFT_25_END_STABILITY=9.7000
PRIMER_RIGHT_25_END_STABILITY=5.4000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_25=167
PRIMER_PAIR_PENALTY_26=1.4178
PRIMER_LEFT_26_PENALTY=0.151542
PRIMER_RIGHT_26_PENALTY=1.266285
PRIMER_LEFT_26_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_26_SEQUENCE=GAACATGTGCACGCATGTAG
PRIMER_LEFT_26=76,20
PRIMER_RIGHT_26=243,20
PRIMER_LEFT_26_TM=60.152
PRIMER_RIGHT_26_TM=58.734
PRIMER_LEFT_26_GC_PERCENT=50.000
PRIMER_RIGHT_26_GC_PERCENT=50.000
PRIMER_LEFT_26_SELF_ANY=5.00
PRIMER_RIGHT_26_SELF_ANY=8.00
PRIMER_LEFT_26_SELF_END=3.00
PRIMER_RIGHT_26_SELF_END=4.00
PRIMER_LEFT_26_END_STABILITY=9.7000
PRIMER_RIGHT_26_END_STABILITY=5.7000
PRIMER_PAIR_26_COMPL_ANY=4.00
PRIMER_PAIR_26_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_26=168
PRIMER_PAIR_PENALTY_27=1.4502
PRIMER_LEFT_27_PENALTY=0.683672
PRIMER_RIGHT_27_PENALTY=0.766499
PRIMER_LEFT_27_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_27_SEQUENCE=TGAACATGTGCACGCATGTA
PRIMER_LEFT_27=88,20
PRIMER_RIGHT_27=244,20
PRIMER_LEFT_27_TM=59.316
PRIMER_RIGHT_27_TM=60.766
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=45.000
PRIMER_LEFT_27_SELF_ANY=5.00
PRIMER_RIGHT_27_SELF_ANY=8.00
PRIMER_LEFT_27_SELF_END=5.00
PRIMER_RIGHT_27_SELF_END=6.00
PRIMER_LEFT_27_END_STABILITY=9.7000
PRIMER_RIGHT_27_END_STABILITY=5.6000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_27=157
PRIMER_PAIR_PENALTY_28=1.4527
PRIMER_LEFT_28_PENALTY=0.775718
PRIMER_RIGHT_28_PENALTY=0.676947
PRIMER_LEFT_28_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_28_SEQUENCE=GTGCACGCATGTAGAGGTTC
PRIMER_LEFT_28=86,20
PRIMER_RIGHT_28=237,20
PRIMER_LEFT_28_TM=60.776
PRIMER_RIGHT_28_TM=59.323
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=55.000
PRIMER_LEFT_28_SELF_ANY=5.00
PRIMER_RIGHT_28_SELF_ANY=6.00
PRIMER_LEFT_28_SELF_END=2.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=9.7000
PRIMER_RIGHT_28_END_STABILITY=7.9000
PRIMER_PAIR_28_COMPL_ANY=4.00
PRIMER_PAIR_28_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_28=152
PRIMER_PAIR_PENALTY_29=1.4527
PRIMER_LEFT_29_PENALTY=0.775718
PRIMER_RIGHT_29_PENALTY=0.676947
PRIMER_LEFT_29_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_29_SEQUENCE=GTGCACGCATGTAGAGGTTC
PRIMER_LEFT_29=87,20
PRIMER_RIGHT_29=237,20
PRIMER_LEFT_29_TM=60.776
PRIMER_RIGHT_29_TM=59.323
PRIMER_LEFT_29_GC_PERCENT=45.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=4.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=3.00
PRIMER_RIGHT_29_SELF_END=1.00
PRIMER_LEFT_29_END_STABILITY=9.4000
PRIMER_RIGHT_29_END_STABILITY=7.9000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_29=151
PRIMER_PAIR_PENALTY_30=1.4620
PRIMER_LEFT_30_PENALTY=0.775718
PRIMER_RIGHT_30_PENALTY=0.686256
PRIMER_LEFT_30_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_30_SEQUENCE=CACGCATGTAGAGGTTCAGG
PRIMER_LEFT_30=86,20
PRIMER_RIGHT_30=234,20
PRIMER_LEFT_30_TM=60.776
PRIMER_RIGHT_30_TM=59.314
PRIMER_LEFT_30_GC_PERCENT=50.000
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=4.00
PRIMER_LEFT_30_SELF_END=2.00
PRIMER_RIGHT_30_SELF_END=0.00
PRIMER_LEFT_30_END_STABILITY=9.7000
PRIMER_RIGHT_30_END_STABILITY=8.2000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_30=149
PRIMER_PAIR_PENALTY_31=1.4620
PRIMER_LEFT_31_PENALTY=0.775718
PRIMER_RIGHT_31_PENALTY=0.686256
PRIMER_LEFT_31_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_31_SEQUENCE=CACGCATGTAGAGGTTCAGG
PRIMER_LEFT_31=87,20
PRIMER_RIGHT_31=234,20
PRIMER_LEFT_31_TM=60.776
PRIMER_RIGHT_31_TM=59.314
PRIMER_LEFT_31_GC_PERCENT=45.000
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=4.00
PRIMER_RIGHT_31_SELF_ANY=4.00
PRIMER_LEFT_31_SELF_END=3.00
PRIMER_RIGHT_31_SELF_END=0.00
PRIMER_LEFT_31_END_STABILITY=9.4000
PRIMER_RIGHT_31_END_STABILITY=8.2000
PRIMER_PAIR_31_COMPL_ANY=4.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_31=148
PRIMER_PAIR_PENALTY_32=1.4620
PRIMER_LEFT_32_PENALTY=0.775718
PRIMER_RIGHT_32_PENALTY=0.686256
PRIMER_LEFT_32_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_32_SEQUENCE=CGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_32=87,20
PRIMER_RIGHT_32=232,20
PRIMER_LEFT_32_TM=60.776
PRIMER_RIGHT_32_TM=59.314
PRIMER_LEFT_32_GC_PERCENT=45.000
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=4.00
PRIMER_RIGHT_32_SELF_ANY=4.00
PRIMER_LEFT_32_SELF_END=3.00
PRIMER_RIGHT_32_SELF_END=2.00
PRIMER_LEFT_32_END_STABILITY=9.4000
PRIMER_RIGHT_32_END_STABILITY=7.9000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_32=146
PRIMER_PAIR_PENALTY_33=1.4620
PRIMER_LEFT_33_PENALTY=0.775718
PRIMER_RIGHT_33_PENALTY=0.686256
PRIMER_LEFT_33_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_33_SEQUENCE=CGCATGTAGAGGTTCAGGTG
PRIMER_LEFT_33=86,20
PRIMER_RIGHT_33=232,20
PRIMER_LEFT_33_TM=60.776
PRIMER_RIGHT_33_TM=59.314
PRIMER_LEFT_33_GC_PERCENT=50.000
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=5.00
PRIMER_RIGHT_33_SELF_ANY=4.00
PRIMER_LEFT_33_SELF_END=2.00
PRIMER_RIGHT_33_SELF_END=2.00
PRIMER_LEFT_33_END_STABILITY=9.7000
PRIMER_RIGHT_33_END_STABILITY=7.9000
PRIMER_PAIR_33_COMPL_ANY=4.00
PRIMER_PAIR_33_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_33=147
PRIMER_PAIR_PENALTY_34=1.4697
PRIMER_LEFT_34_PENALTY=0.151542
PRIMER_RIGHT_34_PENALTY=1.318201
PRIMER_LEFT_34_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_34_SEQUENCE=CATGTGCACGCATGTAGAGG
PRIMER_LEFT_34=76,20
PRIMER_RIGHT_34=240,20
PRIMER_LEFT_34_TM=60.152
PRIMER_RIGHT_34_TM=61.318
PRIMER_LEFT_34_GC_PERCENT=50.000
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=5.00
PRIMER_RIGHT_34_SELF_ANY=6.00
PRIMER_LEFT_34_SELF_END=3.00
PRIMER_RIGHT_34_SELF_END=4.00
PRIMER_LEFT_34_END_STABILITY=9.7000
PRIMER_RIGHT_34_END_STABILITY=7.9000
PRIMER_PAIR_34_COMPL_ANY=4.00
PRIMER_PAIR_34_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_34=165
PRIMER_PAIR_PENALTY_35=1.4884
PRIMER_LEFT_35_PENALTY=0.683672
PRIMER_RIGHT_35_PENALTY=0.804687
PRIMER_LEFT_35_SEQUENCE=GACAGGCAATTTCAAGGCTT
PRIMER_RIGHT_35_SEQUENCE=ATGTGCACGCATGTAGAGGT
PRIMER_LEFT_35=88,20
PRIMER_RIGHT_35=239,20
PRIMER_LEFT_35_TM=59.316
PRIMER_RIGHT_35_TM=59.195
PRIMER_LEFT_35_GC_PERCENT=45.000
PRIMER_RIGHT_35_GC_PERCENT=50.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=6.00
PRIMER_LEFT_35_SELF_END=5.00
PRIMER_RIGHT_35_SELF_END=0.00
PRIMER_LEFT_35_END_STABILITY=9.7000
PRIMER_RIGHT_35_END_STABILITY=7.6000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_35=152
PRIMER_PAIR_PENALTY_36=1.5294
PRIMER_LEFT_36_PENALTY=0.151542
PRIMER_RIGHT_36_PENALTY=1.377815
PRIMER_LEFT_36_SEQUENCE=TGGGTACAAACTGACAGGCA
PRIMER_RIGHT_36_SEQUENCE=GAGGTTCAGGTGGAGAGGG
PRIMER_LEFT_36=76,20
PRIMER_RIGHT_36=224,19
PRIMER_LEFT_36_TM=60.152
PRIMER_RIGHT_36_TM=59.622
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=63.158
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=2.00
PRIMER_LEFT_36_SELF_END=3.00
PRIMER_RIGHT_36_SELF_END=0.00
PRIMER_LEFT_36_END_STABILITY=9.7000
PRIMER_RIGHT_36_END_STABILITY=9.4000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_36=149
PRIMER_PAIR_PENALTY_37=1.5422
PRIMER_LEFT_37_PENALTY=0.775718
PRIMER_RIGHT_37_PENALTY=0.766499
PRIMER_LEFT_37_SEQUENCE=CTGACAGGCAATTTCAAGGC
PRIMER_RIGHT_37_SEQUENCE=TGAACATGTGCACGCATGTA
PRIMER_LEFT_37=86,20
PRIMER_RIGHT_37=244,20
PRIMER_LEFT_37_TM=60.776
PRIMER_RIGHT_37_TM=60.766
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=45.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=8.00
PRIMER_LEFT_37_SELF_END=2.00
PRIMER_RIGHT_37_SELF_END=6.00
PRIMER_LEFT_37_END_STABILITY=9.7000
PRIMER_RIGHT_37_END_STABILITY=5.6000
PRIMER_PAIR_37_COMPL_ANY=5.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_37=159
PRIMER_PAIR_PENALTY_38=1.5422
PRIMER_LEFT_38_PENALTY=0.775718
PRIMER_RIGHT_38_PENALTY=0.766499
PRIMER_LEFT_38_SEQUENCE=TGACAGGCAATTTCAAGGCT
PRIMER_RIGHT_38_SEQUENCE=TGAACATGTGCACGCATGTA
PRIMER_LEFT_38=87,20
PRIMER_RIGHT_38=244,20
PRIMER_LEFT_38_TM=60.776
PRIMER_RIGHT_38_TM=60.766
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=45.000
PRIMER_LEFT_38_SELF_ANY=4.00
PRIMER_RIGHT_38_SELF_ANY=8.00
PRIMER_LEFT_38_SELF_END=3.00
PRIMER_RIGHT_38_SELF_END=6.00
PRIMER_LEFT_38_END_STABILITY=9.4000
PRIMER_RIGHT_38_END_STABILITY=5.6000
PRIMER_PAIR_38_COMPL_ANY=5.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_38=158
PRIMER_PAIR_PENALTY_39=1.5431
PRIMER_LEFT_39_PENALTY=1.210088
PRIMER_RIGHT_39_PENALTY=0.333053
PRIMER_LEFT_39_SEQUENCE=TGACAGGCAATTTCAAGGC
PRIMER_RIGHT_39_SEQUENCE=TGTGCACGCATGTAGAGGTT
PRIMER_LEFT_39=87,19
PRIMER_RIGHT_39=238,20
PRIMER_LEFT_39_TM=59.790
PRIMER_RIGHT_39_TM=60.333
PRIMER_LEFT_39_GC_PERCENT=47.368
PRIMER_RIGHT_39_GC_PERCENT=50.000
PRIMER_LEFT_39_SELF_ANY=4.00
PRIMER_RIGHT_39_SELF_ANY=6.00
PRIMER_LEFT_39_SELF_END=2.00
PRIMER_RIGHT_39_SELF_END=0.00
PRIMER_LEFT_39_END_STABILITY=9.7000
PRIMER_RIGHT_39_END_STABILITY=7.9000
PRIMER_PAIR_39_COMPL_ANY=4.00
PRIMER_PAIR_39_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_39=152
=
MARKER_NAME=08.RHAP34FE8.seq
SEQUENCE=NNNNNNNNNNNNNNNNNNNNCATGCAGTGTGAATATCTCATGTTCTTCAGCTTTTTCCCCTTAGGACCAAGGTATAGCCTTAGGACCACGGATTATCCTTCCATTTTCTTAGAACTCTANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGTGAGTGGCATCTGTCCCACCAGGTCCTTTGAAGAGCTGAGTGAAATGATGTATAAAGTACTTTGCATTGGCAAGGGGGAAGACTCCAGCATGGATCTAGTAGAAGAAATATGAAGGAG
INCLUDED_REGION=20,281
TARGET=119,62,AC
PRIMER_PAIR_PENALTY=0.2190
PRIMER_LEFT_PENALTY=0.201280
PRIMER_RIGHT_PENALTY=0.017711
PRIMER_LEFT_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT=53,20
PRIMER_RIGHT=220,20
PRIMER_LEFT_TM=59.799
PRIMER_RIGHT_TM=59.982
PRIMER_LEFT_GC_PERCENT=50.000
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=7.00
PRIMER_RIGHT_SELF_ANY=6.00
PRIMER_LEFT_SELF_END=5.00
PRIMER_RIGHT_SELF_END=4.00
PRIMER_LEFT_END_STABILITY=7.9000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_MAX_COMPL_ANY=4.00
PRIMER_PAIR_MAX_COMPL_END=4.00
PRIMER_PRODUCT_SIZE=168
PRIMER_PAIR_PENALTY_1=0.2677
PRIMER_LEFT_1_PENALTY=0.249989
PRIMER_RIGHT_1_PENALTY=0.017711
PRIMER_LEFT_1_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_1_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_1=83,20
PRIMER_RIGHT_1=220,20
PRIMER_LEFT_1_TM=59.750
PRIMER_RIGHT_1_TM=59.982
PRIMER_LEFT_1_GC_PERCENT=50.000
PRIMER_RIGHT_1_GC_PERCENT=55.000
PRIMER_LEFT_1_SELF_ANY=7.00
PRIMER_RIGHT_1_SELF_ANY=6.00
PRIMER_LEFT_1_SELF_END=1.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=8.5000
PRIMER_RIGHT_1_END_STABILITY=9.7000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_1=138
PRIMER_PAIR_PENALTY_2=0.3573
PRIMER_LEFT_2_PENALTY=0.201280
PRIMER_RIGHT_2_PENALTY=0.156061
PRIMER_LEFT_2_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_2_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_2=53,20
PRIMER_RIGHT_2=218,20
PRIMER_LEFT_2_TM=59.799
PRIMER_RIGHT_2_TM=59.844
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=55.000
PRIMER_LEFT_2_SELF_ANY=7.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=5.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=7.9000
PRIMER_RIGHT_2_END_STABILITY=8.2000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_2=166
PRIMER_PAIR_PENALTY_3=0.4060
PRIMER_LEFT_3_PENALTY=0.249989
PRIMER_RIGHT_3_PENALTY=0.156061
PRIMER_LEFT_3_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_3_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_3=83,20
PRIMER_RIGHT_3=218,20
PRIMER_LEFT_3_TM=59.750
PRIMER_RIGHT_3_TM=59.844
PRIMER_LEFT_3_GC_PERCENT=50.000
PRIMER_RIGHT_3_GC_PERCENT=55.000
PRIMER_LEFT_3_SELF_ANY=7.00
PRIMER_RIGHT_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=3.00
PRIMER_LEFT_3_END_STABILITY=8.5000
PRIMER_RIGHT_3_END_STABILITY=8.2000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=136
PRIMER_PAIR_PENALTY_4=0.4406
PRIMER_LEFT_4_PENALTY=0.422888
PRIMER_RIGHT_4_PENALTY=0.017711
PRIMER_LEFT_4_SEQUENCE=GCTTTTTCCCCTTAGGACCA
PRIMER_RIGHT_4_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_4=49,20
PRIMER_RIGHT_4=220,20
PRIMER_LEFT_4_TM=60.423
PRIMER_RIGHT_4_TM=59.982
PRIMER_LEFT_4_GC_PERCENT=50.000
PRIMER_RIGHT_4_GC_PERCENT=55.000
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=6.00
PRIMER_LEFT_4_SELF_END=1.00
PRIMER_RIGHT_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=7.9000
PRIMER_RIGHT_4_END_STABILITY=9.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_4=172
PRIMER_PAIR_PENALTY_5=0.5102
PRIMER_LEFT_5_PENALTY=0.201280
PRIMER_RIGHT_5_PENALTY=0.308871
PRIMER_LEFT_5_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_5_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_5=53,20
PRIMER_RIGHT_5=217,20
PRIMER_LEFT_5_TM=59.799
PRIMER_RIGHT_5_TM=59.691
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=55.000
PRIMER_LEFT_5_SELF_ANY=7.00
PRIMER_RIGHT_5_SELF_ANY=5.00
PRIMER_LEFT_5_SELF_END=5.00
PRIMER_RIGHT_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=7.9000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_5=165
PRIMER_PAIR_PENALTY_6=0.5433
PRIMER_LEFT_6_PENALTY=0.525579
PRIMER_RIGHT_6_PENALTY=0.017711
PRIMER_LEFT_6_SEQUENCE=GGACCACGGATTATCCTTCC
PRIMER_RIGHT_6_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_6=82,20
PRIMER_RIGHT_6=220,20
PRIMER_LEFT_6_TM=60.526
PRIMER_RIGHT_6_TM=59.982
PRIMER_LEFT_6_GC_PERCENT=55.000
PRIMER_RIGHT_6_GC_PERCENT=55.000
PRIMER_LEFT_6_SELF_ANY=7.00
PRIMER_RIGHT_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=4.00
PRIMER_LEFT_6_END_STABILITY=8.2000
PRIMER_RIGHT_6_END_STABILITY=9.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=139
PRIMER_PAIR_PENALTY_7=0.5589
PRIMER_LEFT_7_PENALTY=0.249989
PRIMER_RIGHT_7_PENALTY=0.308871
PRIMER_LEFT_7_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_7_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_7=83,20
PRIMER_RIGHT_7=217,20
PRIMER_LEFT_7_TM=59.750
PRIMER_RIGHT_7_TM=59.691
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=55.000
PRIMER_LEFT_7_SELF_ANY=7.00
PRIMER_RIGHT_7_SELF_ANY=5.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=1.00
PRIMER_LEFT_7_END_STABILITY=8.5000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=135
PRIMER_PAIR_PENALTY_8=0.5789
PRIMER_LEFT_8_PENALTY=0.422888
PRIMER_RIGHT_8_PENALTY=0.156061
PRIMER_LEFT_8_SEQUENCE=GCTTTTTCCCCTTAGGACCA
PRIMER_RIGHT_8_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_8=49,20
PRIMER_RIGHT_8=218,20
PRIMER_LEFT_8_TM=60.423
PRIMER_RIGHT_8_TM=59.844
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=55.000
PRIMER_LEFT_8_SELF_ANY=5.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_LEFT_8_SELF_END=1.00
PRIMER_RIGHT_8_SELF_END=3.00
PRIMER_LEFT_8_END_STABILITY=7.9000
PRIMER_RIGHT_8_END_STABILITY=8.2000
PRIMER_PAIR_8_COMPL_ANY=4.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_8=170
PRIMER_PAIR_PENALTY_9=0.6816
PRIMER_LEFT_9_PENALTY=0.525579
PRIMER_RIGHT_9_PENALTY=0.156061
PRIMER_LEFT_9_SEQUENCE=GGACCACGGATTATCCTTCC
PRIMER_RIGHT_9_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_9=82,20
PRIMER_RIGHT_9=218,20
PRIMER_LEFT_9_TM=60.526
PRIMER_RIGHT_9_TM=59.844
PRIMER_LEFT_9_GC_PERCENT=55.000
PRIMER_RIGHT_9_GC_PERCENT=55.000
PRIMER_LEFT_9_SELF_ANY=7.00
PRIMER_RIGHT_9_SELF_ANY=5.00
PRIMER_LEFT_9_SELF_END=3.00
PRIMER_RIGHT_9_SELF_END=3.00
PRIMER_LEFT_9_END_STABILITY=8.2000
PRIMER_RIGHT_9_END_STABILITY=8.2000
PRIMER_PAIR_9_COMPL_ANY=5.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_9=137
PRIMER_PAIR_PENALTY_10=0.7318
PRIMER_LEFT_10_PENALTY=0.422888
PRIMER_RIGHT_10_PENALTY=0.308871
PRIMER_LEFT_10_SEQUENCE=GCTTTTTCCCCTTAGGACCA
PRIMER_RIGHT_10_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_10=49,20
PRIMER_RIGHT_10=217,20
PRIMER_LEFT_10_TM=60.423
PRIMER_RIGHT_10_TM=59.691
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=55.000
PRIMER_LEFT_10_SELF_ANY=5.00
PRIMER_RIGHT_10_SELF_ANY=5.00
PRIMER_LEFT_10_SELF_END=1.00
PRIMER_RIGHT_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=7.9000
PRIMER_RIGHT_10_END_STABILITY=9.4000
PRIMER_PAIR_10_COMPL_ANY=4.00
PRIMER_PAIR_10_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_10=169
PRIMER_PAIR_PENALTY_11=0.8345
PRIMER_LEFT_11_PENALTY=0.525579
PRIMER_RIGHT_11_PENALTY=0.308871
PRIMER_LEFT_11_SEQUENCE=GGACCACGGATTATCCTTCC
PRIMER_RIGHT_11_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_11=82,20
PRIMER_RIGHT_11=217,20
PRIMER_LEFT_11_TM=60.526
PRIMER_RIGHT_11_TM=59.691
PRIMER_LEFT_11_GC_PERCENT=55.000
PRIMER_RIGHT_11_GC_PERCENT=55.000
PRIMER_LEFT_11_SELF_ANY=7.00
PRIMER_RIGHT_11_SELF_ANY=5.00
PRIMER_LEFT_11_SELF_END=3.00
PRIMER_RIGHT_11_SELF_END=1.00
PRIMER_LEFT_11_END_STABILITY=8.2000
PRIMER_RIGHT_11_END_STABILITY=9.4000
PRIMER_PAIR_11_COMPL_ANY=5.00
PRIMER_PAIR_11_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_11=136
PRIMER_PAIR_PENALTY_12=0.9709
PRIMER_LEFT_12_PENALTY=0.201280
PRIMER_RIGHT_12_PENALTY=0.769636
PRIMER_LEFT_12_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_12_SEQUENCE=AAGGACCTGGTGGGACAGAT
PRIMER_LEFT_12=53,20
PRIMER_RIGHT_12=210,20
PRIMER_LEFT_12_TM=59.799
PRIMER_RIGHT_12_TM=60.770
PRIMER_LEFT_12_GC_PERCENT=50.000
PRIMER_RIGHT_12_GC_PERCENT=55.000
PRIMER_LEFT_12_SELF_ANY=7.00
PRIMER_RIGHT_12_SELF_ANY=5.00
PRIMER_LEFT_12_SELF_END=5.00
PRIMER_RIGHT_12_SELF_END=2.00
PRIMER_LEFT_12_END_STABILITY=7.9000
PRIMER_RIGHT_12_END_STABILITY=6.6000
PRIMER_PAIR_12_COMPL_ANY=5.00
PRIMER_PAIR_12_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_12=158
PRIMER_PAIR_PENALTY_13=1.0196
PRIMER_LEFT_13_PENALTY=0.249989
PRIMER_RIGHT_13_PENALTY=0.769636
PRIMER_LEFT_13_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_13_SEQUENCE=AAGGACCTGGTGGGACAGAT
PRIMER_LEFT_13=83,20
PRIMER_RIGHT_13=210,20
PRIMER_LEFT_13_TM=59.750
PRIMER_RIGHT_13_TM=60.770
PRIMER_LEFT_13_GC_PERCENT=50.000
PRIMER_RIGHT_13_GC_PERCENT=55.000
PRIMER_LEFT_13_SELF_ANY=7.00
PRIMER_RIGHT_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=1.00
PRIMER_RIGHT_13_SELF_END=2.00
PRIMER_LEFT_13_END_STABILITY=8.5000
PRIMER_RIGHT_13_END_STABILITY=6.6000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_13=128
PRIMER_PAIR_PENALTY_14=1.0446
PRIMER_LEFT_14_PENALTY=1.026872
PRIMER_RIGHT_14_PENALTY=0.017711
PRIMER_LEFT_14_SEQUENCE=CCACGGATTATCCTTCCATTT
PRIMER_RIGHT_14_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_14=85,21
PRIMER_RIGHT_14=220,20
PRIMER_LEFT_14_TM=60.027
PRIMER_RIGHT_14_TM=59.982
PRIMER_LEFT_14_GC_PERCENT=42.857
PRIMER_RIGHT_14_GC_PERCENT=55.000
PRIMER_LEFT_14_SELF_ANY=7.00
PRIMER_RIGHT_14_SELF_ANY=6.00
PRIMER_LEFT_14_SELF_END=0.00
PRIMER_RIGHT_14_SELF_END=4.00
PRIMER_LEFT_14_END_STABILITY=7.2000
PRIMER_RIGHT_14_END_STABILITY=9.7000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_14=136
PRIMER_PAIR_PENALTY_15=1.0492
PRIMER_LEFT_15_PENALTY=1.031536
PRIMER_RIGHT_15_PENALTY=0.017711
PRIMER_LEFT_15_SEQUENCE=GACCACGGATTATCCTTCCAT
PRIMER_RIGHT_15_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_15=83,21
PRIMER_RIGHT_15=220,20
PRIMER_LEFT_15_TM=60.032
PRIMER_RIGHT_15_TM=59.982
PRIMER_LEFT_15_GC_PERCENT=47.619
PRIMER_RIGHT_15_GC_PERCENT=55.000
PRIMER_LEFT_15_SELF_ANY=7.00
PRIMER_RIGHT_15_SELF_ANY=6.00
PRIMER_LEFT_15_SELF_END=2.00
PRIMER_RIGHT_15_SELF_END=4.00
PRIMER_LEFT_15_END_STABILITY=8.1000
PRIMER_RIGHT_15_END_STABILITY=9.7000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_15=138
PRIMER_PAIR_PENALTY_16=1.1169
PRIMER_LEFT_16_PENALTY=1.099227
PRIMER_RIGHT_16_PENALTY=0.017711
PRIMER_LEFT_16_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
PRIMER_RIGHT_16_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_16=46,20
PRIMER_RIGHT_16=220,20
PRIMER_LEFT_16_TM=58.901
PRIMER_RIGHT_16_TM=59.982
PRIMER_LEFT_16_GC_PERCENT=45.000
PRIMER_RIGHT_16_GC_PERCENT=55.000
PRIMER_LEFT_16_SELF_ANY=5.00
PRIMER_RIGHT_16_SELF_ANY=6.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=4.00
PRIMER_LEFT_16_END_STABILITY=7.2000
PRIMER_RIGHT_16_END_STABILITY=9.7000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_16=175
PRIMER_PAIR_PENALTY_17=1.1538
PRIMER_LEFT_17_PENALTY=1.136051
PRIMER_RIGHT_17_PENALTY=0.017711
PRIMER_LEFT_17_SEQUENCE=GGTATAGCCTTAGGACCACGG
PRIMER_RIGHT_17_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_17=70,21
PRIMER_RIGHT_17=220,20
PRIMER_LEFT_17_TM=59.864
PRIMER_RIGHT_17_TM=59.982
PRIMER_LEFT_17_GC_PERCENT=57.143
PRIMER_RIGHT_17_GC_PERCENT=55.000
PRIMER_LEFT_17_SELF_ANY=5.00
PRIMER_RIGHT_17_SELF_ANY=6.00
PRIMER_LEFT_17_SELF_END=3.00
PRIMER_RIGHT_17_SELF_END=4.00
PRIMER_LEFT_17_END_STABILITY=9.9000
PRIMER_RIGHT_17_END_STABILITY=9.7000
PRIMER_PAIR_17_COMPL_ANY=4.00
PRIMER_PAIR_17_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_17=151
PRIMER_PAIR_PENALTY_18=1.1829
PRIMER_LEFT_18_PENALTY=1.026872
PRIMER_RIGHT_18_PENALTY=0.156061
PRIMER_LEFT_18_SEQUENCE=CCACGGATTATCCTTCCATTT
PRIMER_RIGHT_18_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_18=85,21
PRIMER_RIGHT_18=218,20
PRIMER_LEFT_18_TM=60.027
PRIMER_RIGHT_18_TM=59.844
PRIMER_LEFT_18_GC_PERCENT=42.857
PRIMER_RIGHT_18_GC_PERCENT=55.000
PRIMER_LEFT_18_SELF_ANY=7.00
PRIMER_RIGHT_18_SELF_ANY=5.00
PRIMER_LEFT_18_SELF_END=0.00
PRIMER_RIGHT_18_SELF_END=3.00
PRIMER_LEFT_18_END_STABILITY=7.2000
PRIMER_RIGHT_18_END_STABILITY=8.2000
PRIMER_PAIR_18_COMPL_ANY=5.00
PRIMER_PAIR_18_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_18=134
PRIMER_PAIR_PENALTY_19=1.1876
PRIMER_LEFT_19_PENALTY=1.031536
PRIMER_RIGHT_19_PENALTY=0.156061
PRIMER_LEFT_19_SEQUENCE=GACCACGGATTATCCTTCCAT
PRIMER_RIGHT_19_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_19=83,21
PRIMER_RIGHT_19=218,20
PRIMER_LEFT_19_TM=60.032
PRIMER_RIGHT_19_TM=59.844
PRIMER_LEFT_19_GC_PERCENT=47.619
PRIMER_RIGHT_19_GC_PERCENT=55.000
PRIMER_LEFT_19_SELF_ANY=7.00
PRIMER_RIGHT_19_SELF_ANY=5.00
PRIMER_LEFT_19_SELF_END=2.00
PRIMER_RIGHT_19_SELF_END=3.00
PRIMER_LEFT_19_END_STABILITY=8.1000
PRIMER_RIGHT_19_END_STABILITY=8.2000
PRIMER_PAIR_19_COMPL_ANY=5.00
PRIMER_PAIR_19_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_19=136
PRIMER_PAIR_PENALTY_20=1.1925
PRIMER_LEFT_20_PENALTY=0.422888
PRIMER_RIGHT_20_PENALTY=0.769636
PRIMER_LEFT_20_SEQUENCE=GCTTTTTCCCCTTAGGACCA
PRIMER_RIGHT_20_SEQUENCE=AAGGACCTGGTGGGACAGAT
PRIMER_LEFT_20=49,20
PRIMER_RIGHT_20=210,20
PRIMER_LEFT_20_TM=60.423
PRIMER_RIGHT_20_TM=60.770
PRIMER_LEFT_20_GC_PERCENT=50.000
PRIMER_RIGHT_20_GC_PERCENT=55.000
PRIMER_LEFT_20_SELF_ANY=5.00
PRIMER_RIGHT_20_SELF_ANY=5.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=2.00
PRIMER_LEFT_20_END_STABILITY=7.9000
PRIMER_RIGHT_20_END_STABILITY=6.6000
PRIMER_PAIR_20_COMPL_ANY=5.00
PRIMER_PAIR_20_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_20=162
PRIMER_PAIR_PENALTY_21=1.2017
PRIMER_LEFT_21_PENALTY=1.184005
PRIMER_RIGHT_21_PENALTY=0.017711
PRIMER_LEFT_21_SEQUENCE=TTTCCCCTTAGGACCAAGGTA
PRIMER_RIGHT_21_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_21=53,21
PRIMER_RIGHT_21=220,20
PRIMER_LEFT_21_TM=59.816
PRIMER_RIGHT_21_TM=59.982
PRIMER_LEFT_21_GC_PERCENT=47.619
PRIMER_RIGHT_21_GC_PERCENT=55.000
PRIMER_LEFT_21_SELF_ANY=7.00
PRIMER_RIGHT_21_SELF_ANY=6.00
PRIMER_LEFT_21_SELF_END=3.00
PRIMER_RIGHT_21_SELF_END=4.00
PRIMER_LEFT_21_END_STABILITY=6.9000
PRIMER_RIGHT_21_END_STABILITY=9.7000
PRIMER_PAIR_21_COMPL_ANY=4.00
PRIMER_PAIR_21_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_21=168
PRIMER_PAIR_PENALTY_22=1.2054
PRIMER_LEFT_22_PENALTY=1.187663
PRIMER_RIGHT_22_PENALTY=0.017711
PRIMER_LEFT_22_SEQUENCE=GCCTTAGGACCACGGATTATC
PRIMER_RIGHT_22_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_22=76,21
PRIMER_RIGHT_22=220,20
PRIMER_LEFT_22_TM=59.812
PRIMER_RIGHT_22_TM=59.982
PRIMER_LEFT_22_GC_PERCENT=52.381
PRIMER_RIGHT_22_GC_PERCENT=55.000
PRIMER_LEFT_22_SELF_ANY=5.00
PRIMER_RIGHT_22_SELF_ANY=6.00
PRIMER_LEFT_22_SELF_END=5.00
PRIMER_RIGHT_22_SELF_END=4.00
PRIMER_LEFT_22_END_STABILITY=5.9000
PRIMER_RIGHT_22_END_STABILITY=9.7000
PRIMER_PAIR_22_COMPL_ANY=4.00
PRIMER_PAIR_22_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_22=145
PRIMER_PAIR_PENALTY_23=1.2553
PRIMER_LEFT_23_PENALTY=1.099227
PRIMER_RIGHT_23_PENALTY=0.156061
PRIMER_LEFT_23_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
PRIMER_RIGHT_23_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_23=46,20
PRIMER_RIGHT_23=218,20
PRIMER_LEFT_23_TM=58.901
PRIMER_RIGHT_23_TM=59.844
PRIMER_LEFT_23_GC_PERCENT=45.000
PRIMER_RIGHT_23_GC_PERCENT=55.000
PRIMER_LEFT_23_SELF_ANY=5.00
PRIMER_RIGHT_23_SELF_ANY=5.00
PRIMER_LEFT_23_SELF_END=3.00
PRIMER_RIGHT_23_SELF_END=3.00
PRIMER_LEFT_23_END_STABILITY=7.2000
PRIMER_RIGHT_23_END_STABILITY=8.2000
PRIMER_PAIR_23_COMPL_ANY=4.00
PRIMER_PAIR_23_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_23=173
PRIMER_PAIR_PENALTY_24=1.2554
PRIMER_LEFT_24_PENALTY=0.201280
PRIMER_RIGHT_24_PENALTY=1.054163
PRIMER_LEFT_24_SEQUENCE=TTTCCCCTTAGGACCAAGGT
PRIMER_RIGHT_24_SEQUENCE=AAAGGACCTGGTGGGACAG
PRIMER_LEFT_24=53,20
PRIMER_RIGHT_24=211,19
PRIMER_LEFT_24_TM=59.799
PRIMER_RIGHT_24_TM=59.946
PRIMER_LEFT_24_GC_PERCENT=50.000
PRIMER_RIGHT_24_GC_PERCENT=57.895
PRIMER_LEFT_24_SELF_ANY=7.00
PRIMER_RIGHT_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=5.00
PRIMER_RIGHT_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=7.9000
PRIMER_RIGHT_24_END_STABILITY=6.4000
PRIMER_PAIR_24_COMPL_ANY=5.00
PRIMER_PAIR_24_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_24=159
PRIMER_PAIR_PENALTY_25=1.2921
PRIMER_LEFT_25_PENALTY=1.136051
PRIMER_RIGHT_25_PENALTY=0.156061
PRIMER_LEFT_25_SEQUENCE=GGTATAGCCTTAGGACCACGG
PRIMER_RIGHT_25_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_25=70,21
PRIMER_RIGHT_25=218,20
PRIMER_LEFT_25_TM=59.864
PRIMER_RIGHT_25_TM=59.844
PRIMER_LEFT_25_GC_PERCENT=57.143
PRIMER_RIGHT_25_GC_PERCENT=55.000
PRIMER_LEFT_25_SELF_ANY=5.00
PRIMER_RIGHT_25_SELF_ANY=5.00
PRIMER_LEFT_25_SELF_END=3.00
PRIMER_RIGHT_25_SELF_END=3.00
PRIMER_LEFT_25_END_STABILITY=9.9000
PRIMER_RIGHT_25_END_STABILITY=8.2000
PRIMER_PAIR_25_COMPL_ANY=4.00
PRIMER_PAIR_25_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_25=149
PRIMER_PAIR_PENALTY_26=1.2952
PRIMER_LEFT_26_PENALTY=0.525579
PRIMER_RIGHT_26_PENALTY=0.769636
PRIMER_LEFT_26_SEQUENCE=GGACCACGGATTATCCTTCC
PRIMER_RIGHT_26_SEQUENCE=AAGGACCTGGTGGGACAGAT
PRIMER_LEFT_26=82,20
PRIMER_RIGHT_26=210,20
PRIMER_LEFT_26_TM=60.526
PRIMER_RIGHT_26_TM=60.770
PRIMER_LEFT_26_GC_PERCENT=55.000
PRIMER_RIGHT_26_GC_PERCENT=55.000
PRIMER_LEFT_26_SELF_ANY=7.00
PRIMER_RIGHT_26_SELF_ANY=5.00
PRIMER_LEFT_26_SELF_END=3.00
PRIMER_RIGHT_26_SELF_END=2.00
PRIMER_LEFT_26_END_STABILITY=8.2000
PRIMER_RIGHT_26_END_STABILITY=6.6000
PRIMER_PAIR_26_COMPL_ANY=5.00
PRIMER_PAIR_26_COMPL_END=4.00
PRIMER_PRODUCT_SIZE_26=129
PRIMER_PAIR_PENALTY_27=1.3030
PRIMER_LEFT_27_PENALTY=1.285273
PRIMER_RIGHT_27_PENALTY=0.017711
PRIMER_LEFT_27_SEQUENCE=CCACGGATTATCCTTCCATT
PRIMER_RIGHT_27_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_27=85,20
PRIMER_RIGHT_27=220,20
PRIMER_LEFT_27_TM=58.715
PRIMER_RIGHT_27_TM=59.982
PRIMER_LEFT_27_GC_PERCENT=45.000
PRIMER_RIGHT_27_GC_PERCENT=55.000
PRIMER_LEFT_27_SELF_ANY=7.00
PRIMER_RIGHT_27_SELF_ANY=6.00
PRIMER_LEFT_27_SELF_END=1.00
PRIMER_RIGHT_27_SELF_END=4.00
PRIMER_LEFT_27_END_STABILITY=8.4000
PRIMER_RIGHT_27_END_STABILITY=9.7000
PRIMER_PAIR_27_COMPL_ANY=5.00
PRIMER_PAIR_27_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_27=136
PRIMER_PAIR_PENALTY_28=1.3042
PRIMER_LEFT_28_PENALTY=0.249989
PRIMER_RIGHT_28_PENALTY=1.054163
PRIMER_LEFT_28_SEQUENCE=GACCACGGATTATCCTTCCA
PRIMER_RIGHT_28_SEQUENCE=AAAGGACCTGGTGGGACAG
PRIMER_LEFT_28=83,20
PRIMER_RIGHT_28=211,19
PRIMER_LEFT_28_TM=59.750
PRIMER_RIGHT_28_TM=59.946
PRIMER_LEFT_28_GC_PERCENT=50.000
PRIMER_RIGHT_28_GC_PERCENT=57.895
PRIMER_LEFT_28_SELF_ANY=7.00
PRIMER_RIGHT_28_SELF_ANY=5.00
PRIMER_LEFT_28_SELF_END=1.00
PRIMER_RIGHT_28_SELF_END=1.00
PRIMER_LEFT_28_END_STABILITY=8.5000
PRIMER_RIGHT_28_END_STABILITY=6.4000
PRIMER_PAIR_28_COMPL_ANY=5.00
PRIMER_PAIR_28_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_28=129
PRIMER_PAIR_PENALTY_29=1.3055
PRIMER_LEFT_29_PENALTY=1.287819
PRIMER_RIGHT_29_PENALTY=0.017711
PRIMER_LEFT_29_SEQUENCE=AGCTTTTTCCCCTTAGGACC
PRIMER_RIGHT_29_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_29=48,20
PRIMER_RIGHT_29=220,20
PRIMER_LEFT_29_TM=58.712
PRIMER_RIGHT_29_TM=59.982
PRIMER_LEFT_29_GC_PERCENT=50.000
PRIMER_RIGHT_29_GC_PERCENT=55.000
PRIMER_LEFT_29_SELF_ANY=5.00
PRIMER_RIGHT_29_SELF_ANY=6.00
PRIMER_LEFT_29_SELF_END=3.00
PRIMER_RIGHT_29_SELF_END=4.00
PRIMER_LEFT_29_END_STABILITY=9.1000
PRIMER_RIGHT_29_END_STABILITY=9.7000
PRIMER_PAIR_29_COMPL_ANY=4.00
PRIMER_PAIR_29_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_29=173
PRIMER_PAIR_PENALTY_30=1.3059
PRIMER_LEFT_30_PENALTY=1.288173
PRIMER_RIGHT_30_PENALTY=0.017711
PRIMER_LEFT_30_SEQUENCE=TCAGCTTTTTCCCCTTAGGAC
PRIMER_RIGHT_30_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_30=46,21
PRIMER_RIGHT_30=220,20
PRIMER_LEFT_30_TM=59.712
PRIMER_RIGHT_30_TM=59.982
PRIMER_LEFT_30_GC_PERCENT=47.619
PRIMER_RIGHT_30_GC_PERCENT=55.000
PRIMER_LEFT_30_SELF_ANY=5.00
PRIMER_RIGHT_30_SELF_ANY=6.00
PRIMER_LEFT_30_SELF_END=1.00
PRIMER_RIGHT_30_SELF_END=4.00
PRIMER_LEFT_30_END_STABILITY=7.6000
PRIMER_RIGHT_30_END_STABILITY=9.7000
PRIMER_PAIR_30_COMPL_ANY=4.00
PRIMER_PAIR_30_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_30=175
PRIMER_PAIR_PENALTY_31=1.3357
PRIMER_LEFT_31_PENALTY=1.026872
PRIMER_RIGHT_31_PENALTY=0.308871
PRIMER_LEFT_31_SEQUENCE=CCACGGATTATCCTTCCATTT
PRIMER_RIGHT_31_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_31=85,21
PRIMER_RIGHT_31=217,20
PRIMER_LEFT_31_TM=60.027
PRIMER_RIGHT_31_TM=59.691
PRIMER_LEFT_31_GC_PERCENT=42.857
PRIMER_RIGHT_31_GC_PERCENT=55.000
PRIMER_LEFT_31_SELF_ANY=7.00
PRIMER_RIGHT_31_SELF_ANY=5.00
PRIMER_LEFT_31_SELF_END=0.00
PRIMER_RIGHT_31_SELF_END=1.00
PRIMER_LEFT_31_END_STABILITY=7.2000
PRIMER_RIGHT_31_END_STABILITY=9.4000
PRIMER_PAIR_31_COMPL_ANY=5.00
PRIMER_PAIR_31_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_31=133
PRIMER_PAIR_PENALTY_32=1.3393
PRIMER_LEFT_32_PENALTY=1.183236
PRIMER_RIGHT_32_PENALTY=0.156061
PRIMER_LEFT_32_SEQUENCE=TTCAGCTTTTTCCCCTTAGGA
PRIMER_RIGHT_32_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_32=45,21
PRIMER_RIGHT_32=218,20
PRIMER_LEFT_32_TM=60.183
PRIMER_RIGHT_32_TM=59.844
PRIMER_LEFT_32_GC_PERCENT=42.857
PRIMER_RIGHT_32_GC_PERCENT=55.000
PRIMER_LEFT_32_SELF_ANY=5.00
PRIMER_RIGHT_32_SELF_ANY=5.00
PRIMER_LEFT_32_SELF_END=3.00
PRIMER_RIGHT_32_SELF_END=3.00
PRIMER_LEFT_32_END_STABILITY=7.2000
PRIMER_RIGHT_32_END_STABILITY=8.2000
PRIMER_PAIR_32_COMPL_ANY=4.00
PRIMER_PAIR_32_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_32=174
PRIMER_PAIR_PENALTY_33=1.3401
PRIMER_LEFT_33_PENALTY=1.184005
PRIMER_RIGHT_33_PENALTY=0.156061
PRIMER_LEFT_33_SEQUENCE=TTTCCCCTTAGGACCAAGGTA
PRIMER_RIGHT_33_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_33=53,21
PRIMER_RIGHT_33=218,20
PRIMER_LEFT_33_TM=59.816
PRIMER_RIGHT_33_TM=59.844
PRIMER_LEFT_33_GC_PERCENT=47.619
PRIMER_RIGHT_33_GC_PERCENT=55.000
PRIMER_LEFT_33_SELF_ANY=7.00
PRIMER_RIGHT_33_SELF_ANY=5.00
PRIMER_LEFT_33_SELF_END=3.00
PRIMER_RIGHT_33_SELF_END=3.00
PRIMER_LEFT_33_END_STABILITY=6.9000
PRIMER_RIGHT_33_END_STABILITY=8.2000
PRIMER_PAIR_33_COMPL_ANY=5.00
PRIMER_PAIR_33_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_33=166
PRIMER_PAIR_PENALTY_34=1.3404
PRIMER_LEFT_34_PENALTY=1.031536
PRIMER_RIGHT_34_PENALTY=0.308871
PRIMER_LEFT_34_SEQUENCE=GACCACGGATTATCCTTCCAT
PRIMER_RIGHT_34_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_34=83,21
PRIMER_RIGHT_34=217,20
PRIMER_LEFT_34_TM=60.032
PRIMER_RIGHT_34_TM=59.691
PRIMER_LEFT_34_GC_PERCENT=47.619
PRIMER_RIGHT_34_GC_PERCENT=55.000
PRIMER_LEFT_34_SELF_ANY=7.00
PRIMER_RIGHT_34_SELF_ANY=5.00
PRIMER_LEFT_34_SELF_END=2.00
PRIMER_RIGHT_34_SELF_END=1.00
PRIMER_LEFT_34_END_STABILITY=8.1000
PRIMER_RIGHT_34_END_STABILITY=9.4000
PRIMER_PAIR_34_COMPL_ANY=5.00
PRIMER_PAIR_34_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_34=135
PRIMER_PAIR_PENALTY_35=1.3437
PRIMER_LEFT_35_PENALTY=1.187663
PRIMER_RIGHT_35_PENALTY=0.156061
PRIMER_LEFT_35_SEQUENCE=GCCTTAGGACCACGGATTATC
PRIMER_RIGHT_35_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_35=76,21
PRIMER_RIGHT_35=218,20
PRIMER_LEFT_35_TM=59.812
PRIMER_RIGHT_35_TM=59.844
PRIMER_LEFT_35_GC_PERCENT=52.381
PRIMER_RIGHT_35_GC_PERCENT=55.000
PRIMER_LEFT_35_SELF_ANY=5.00
PRIMER_RIGHT_35_SELF_ANY=5.00
PRIMER_LEFT_35_SELF_END=5.00
PRIMER_RIGHT_35_SELF_END=3.00
PRIMER_LEFT_35_END_STABILITY=5.9000
PRIMER_RIGHT_35_END_STABILITY=8.2000
PRIMER_PAIR_35_COMPL_ANY=4.00
PRIMER_PAIR_35_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_35=143
PRIMER_PAIR_PENALTY_36=1.3471
PRIMER_LEFT_36_PENALTY=1.329419
PRIMER_RIGHT_36_PENALTY=0.017711
PRIMER_LEFT_36_SEQUENCE=AGCCTTAGGACCACGGATTA
PRIMER_RIGHT_36_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_36=75,20
PRIMER_RIGHT_36=220,20
PRIMER_LEFT_36_TM=58.671
PRIMER_RIGHT_36_TM=59.982
PRIMER_LEFT_36_GC_PERCENT=50.000
PRIMER_RIGHT_36_GC_PERCENT=55.000
PRIMER_LEFT_36_SELF_ANY=5.00
PRIMER_RIGHT_36_SELF_ANY=6.00
PRIMER_LEFT_36_SELF_END=2.00
PRIMER_RIGHT_36_SELF_END=4.00
PRIMER_LEFT_36_END_STABILITY=5.9000
PRIMER_RIGHT_36_END_STABILITY=9.7000
PRIMER_PAIR_36_COMPL_ANY=4.00
PRIMER_PAIR_36_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_36=146
PRIMER_PAIR_PENALTY_37=1.3471
PRIMER_LEFT_37_PENALTY=1.329419
PRIMER_RIGHT_37_PENALTY=0.017711
PRIMER_LEFT_37_SEQUENCE=TAGCCTTAGGACCACGGATT
PRIMER_RIGHT_37_SEQUENCE=CAGCTCTTCAAAGGACCTGG
PRIMER_LEFT_37=74,20
PRIMER_RIGHT_37=220,20
PRIMER_LEFT_37_TM=58.671
PRIMER_RIGHT_37_TM=59.982
PRIMER_LEFT_37_GC_PERCENT=50.000
PRIMER_RIGHT_37_GC_PERCENT=55.000
PRIMER_LEFT_37_SELF_ANY=5.00
PRIMER_RIGHT_37_SELF_ANY=6.00
PRIMER_LEFT_37_SELF_END=1.00
PRIMER_RIGHT_37_SELF_END=4.00
PRIMER_LEFT_37_END_STABILITY=8.1000
PRIMER_RIGHT_37_END_STABILITY=9.7000
PRIMER_PAIR_37_COMPL_ANY=4.00
PRIMER_PAIR_37_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_37=147
PRIMER_PAIR_PENALTY_38=1.4081
PRIMER_LEFT_38_PENALTY=1.099227
PRIMER_RIGHT_38_PENALTY=0.308871
PRIMER_LEFT_38_SEQUENCE=TCAGCTTTTTCCCCTTAGGA
PRIMER_RIGHT_38_SEQUENCE=CTCTTCAAAGGACCTGGTGG
PRIMER_LEFT_38=46,20
PRIMER_RIGHT_38=217,20
PRIMER_LEFT_38_TM=58.901
PRIMER_RIGHT_38_TM=59.691
PRIMER_LEFT_38_GC_PERCENT=45.000
PRIMER_RIGHT_38_GC_PERCENT=55.000
PRIMER_LEFT_38_SELF_ANY=5.00
PRIMER_RIGHT_38_SELF_ANY=5.00
PRIMER_LEFT_38_SELF_END=3.00
PRIMER_RIGHT_38_SELF_END=1.00
PRIMER_LEFT_38_END_STABILITY=7.2000
PRIMER_RIGHT_38_END_STABILITY=9.4000
PRIMER_PAIR_38_COMPL_ANY=4.00
PRIMER_PAIR_38_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_38=172
PRIMER_PAIR_PENALTY_39=1.4413
PRIMER_LEFT_39_PENALTY=1.285273
PRIMER_RIGHT_39_PENALTY=0.156061
PRIMER_LEFT_39_SEQUENCE=CCACGGATTATCCTTCCATT
PRIMER_RIGHT_39_SEQUENCE=GCTCTTCAAAGGACCTGGTG
PRIMER_LEFT_39=85,20
PRIMER_RIGHT_39=218,20
PRIMER_LEFT_39_TM=58.715
PRIMER_RIGHT_39_TM=59.844
PRIMER_LEFT_39_GC_PERCENT=45.000
PRIMER_RIGHT_39_GC_PERCENT=55.000
PRIMER_LEFT_39_SELF_ANY=7.00
PRIMER_RIGHT_39_SELF_ANY=5.00
PRIMER_LEFT_39_SELF_END=1.00
PRIMER_RIGHT_39_SELF_END=3.00
PRIMER_LEFT_39_END_STABILITY=8.4000
PRIMER_RIGHT_39_END_STABILITY=8.2000
PRIMER_PAIR_39_COMPL_ANY=5.00
PRIMER_PAIR_39_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_39=134
=
PRIMER_NUM_RETURN=8
MARKER_NAME=09.RHAP33FA9.seq
SEQUENCE=GCCTTGGGATTTTACTTATTGAGACATCCTGTTACCATGTAGGAGTTTAGAGAGGTTTCATTTTCTCTGCTAAGTAATGAGTTCAAAGCCAGGAAATCATCTCAGAGACAGCAGGCAGCAATAAGAAACACATTTTATTTCAGTTTATGAANNNNNNNNNNNNNNNNNNAGGGGGGAGGGAGGGAGGGACAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCNNNNNNNNNNNNNNNNNCANNNCNCNCTGNAGCANAANCNCCCTGCAAGNGCCGNCGNNGTNNCNGCNNTNNNNTCCCCCNGCNGGNGNNCCGNGCNCCCNNCCGGCGNNNCNNCNTGCNGNNG
INCLUDED_REGION=0,371
TARGET=151,18,AC
TARGET=192,53,GA
TARGET=248,16,AG
PRIMER_PAIR_PENALTY=8.2998
PRIMER_LEFT_PENALTY=4.294975
PRIMER_RIGHT_PENALTY=4.004782
PRIMER_LEFT_SEQUENCE=TGAGACATCCTGTTACCATGTAGG
PRIMER_RIGHT_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT=19,24
PRIMER_RIGHT=191,18
PRIMER_LEFT_TM=60.295
PRIMER_RIGHT_TM=62.005
PRIMER_LEFT_GC_PERCENT=45.833
PRIMER_RIGHT_GC_PERCENT=72.222
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=1.00
PRIMER_LEFT_SELF_END=2.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=6.9000
PRIMER_RIGHT_END_STABILITY=9.4000
PRIMER_PAIR_MAX_COMPL_ANY=4.00
PRIMER_PAIR_MAX_COMPL_END=3.00
PRIMER_PRODUCT_SIZE=173
PRIMER_PAIR_PENALTY_1=8.3060
PRIMER_LEFT_1_PENALTY=4.301260
PRIMER_RIGHT_1_PENALTY=4.004782
PRIMER_LEFT_1_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCCA
PRIMER_RIGHT_1_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_1=67,24
PRIMER_RIGHT_1=191,18
PRIMER_LEFT_1_TM=60.301
PRIMER_RIGHT_1_TM=62.005
PRIMER_LEFT_1_GC_PERCENT=37.500
PRIMER_RIGHT_1_GC_PERCENT=72.222
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=1.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=9.7000
PRIMER_RIGHT_1_END_STABILITY=9.4000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_1=125
PRIMER_PAIR_PENALTY_2=8.5894
PRIMER_LEFT_2_PENALTY=4.584629
PRIMER_RIGHT_2_PENALTY=4.004782
PRIMER_LEFT_2_SEQUENCE=GAGACATCCTGTTACCATGTAGGA
PRIMER_RIGHT_2_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_2=20,24
PRIMER_RIGHT_2=191,18
PRIMER_LEFT_2_TM=59.415
PRIMER_RIGHT_2_TM=62.005
PRIMER_LEFT_2_GC_PERCENT=45.833
PRIMER_RIGHT_2_GC_PERCENT=72.222
PRIMER_LEFT_2_SELF_ANY=4.00
PRIMER_RIGHT_2_SELF_ANY=1.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=7.2000
PRIMER_RIGHT_2_END_STABILITY=9.4000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_2=172
PRIMER_PAIR_PENALTY_3=8.9567
PRIMER_LEFT_3_PENALTY=4.951910
PRIMER_RIGHT_3_PENALTY=4.004782
PRIMER_LEFT_3_SEQUENCE=TTGAGACATCCTGTTACCATGT
PRIMER_RIGHT_3_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_3=18,22
PRIMER_RIGHT_3=191,18
PRIMER_LEFT_3_TM=57.048
PRIMER_RIGHT_3_TM=62.005
PRIMER_LEFT_3_GC_PERCENT=40.909
PRIMER_RIGHT_3_GC_PERCENT=72.222
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=1.00
PRIMER_LEFT_3_SELF_END=3.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_LEFT_3_END_STABILITY=6.6000
PRIMER_RIGHT_3_END_STABILITY=9.4000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=174
PRIMER_PAIR_PENALTY_4=9.1359
PRIMER_LEFT_4_PENALTY=5.131103
PRIMER_RIGHT_4_PENALTY=4.004782
PRIMER_LEFT_4_SEQUENCE=AGAGAGGTTTCATTTTCTCTGCT
PRIMER_RIGHT_4_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_4=48,23
PRIMER_RIGHT_4=191,18
PRIMER_LEFT_4_TM=57.869
PRIMER_RIGHT_4_TM=62.005
PRIMER_LEFT_4_GC_PERCENT=39.130
PRIMER_RIGHT_4_GC_PERCENT=72.222
PRIMER_LEFT_4_SELF_ANY=5.00
PRIMER_RIGHT_4_SELF_ANY=1.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=8.2000
PRIMER_RIGHT_4_END_STABILITY=9.4000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=144
PRIMER_PAIR_PENALTY_5=9.2025
PRIMER_LEFT_5_PENALTY=5.197674
PRIMER_RIGHT_5_PENALTY=4.004782
PRIMER_LEFT_5_SEQUENCE=TGCTAAGTAATGAGTTCAAAGCC
PRIMER_RIGHT_5_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_5=67,23
PRIMER_RIGHT_5=191,18
PRIMER_LEFT_5_TM=57.802
PRIMER_RIGHT_5_TM=62.005
PRIMER_LEFT_5_GC_PERCENT=39.130
PRIMER_RIGHT_5_GC_PERCENT=72.222
PRIMER_LEFT_5_SELF_ANY=4.00
PRIMER_RIGHT_5_SELF_ANY=1.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=9.7000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=3.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=125
PRIMER_PAIR_PENALTY_6=9.2792
PRIMER_LEFT_6_PENALTY=5.274423
PRIMER_RIGHT_6_PENALTY=4.004782
PRIMER_LEFT_6_SEQUENCE=GACATCCTGTTACCATGTAGGAGTT
PRIMER_RIGHT_6_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_6=22,25
PRIMER_RIGHT_6=191,18
PRIMER_LEFT_6_TM=59.726
PRIMER_RIGHT_6_TM=62.005
PRIMER_LEFT_6_GC_PERCENT=44.000
PRIMER_RIGHT_6_GC_PERCENT=72.222
PRIMER_LEFT_6_SELF_ANY=4.00
PRIMER_RIGHT_6_SELF_ANY=1.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.4000
PRIMER_RIGHT_6_END_STABILITY=9.4000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_6=170
PRIMER_PAIR_PENALTY_7=9.2850
PRIMER_LEFT_7_PENALTY=5.280169
PRIMER_RIGHT_7_PENALTY=4.004782
PRIMER_LEFT_7_SEQUENCE=CTGCTAAGTAATGAGTTCAAAGCC
PRIMER_RIGHT_7_SEQUENCE=CTGTCCCTCCCTCCCTCC
PRIMER_LEFT_7=66,24
PRIMER_RIGHT_7=191,18
PRIMER_LEFT_7_TM=58.720
PRIMER_RIGHT_7_TM=62.005
PRIMER_LEFT_7_GC_PERCENT=41.667
PRIMER_RIGHT_7_GC_PERCENT=72.222
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=1.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=126
=
MARKER_NAME=09.RHAP34FA9.seq
SEQUENCE=NNNNNNNNNNNNNNNNNNNNTGATCAGGATTTCAGTGCTAATATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCCCCTTTGGTAACTCCTGTTGTACAAGGGCTCCAAAAAGCAGGAAAGAATCAGAAACAGCCCCTGTTCCCTCTGTGAGGAAACCTACAAAAATGCAAGCAACACAAATATAATGTGTATACAGATAGCTTAGGTTAGATTCAAGCAGGCTCTTGTATTTTCAGCTCAGTCTCAGTGTAGCACCTGTAAATGCAGGTTGATTGAGGTTTATTTAT
INCLUDED_REGION=20,280
TARGET=44,42,CA
PRIMER_PAIR_PENALTY=7.2009
PRIMER_LEFT_PENALTY=6.971746
PRIMER_RIGHT_PENALTY=0.229181
PRIMER_LEFT_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_SEQUENCE=TTCCTCACAGAGGGAACAGG
PRIMER_LEFT=20,24
PRIMER_RIGHT=166,20
PRIMER_LEFT_TM=57.028
PRIMER_RIGHT_TM=60.229
PRIMER_LEFT_GC_PERCENT=33.333
PRIMER_RIGHT_GC_PERCENT=55.000
PRIMER_LEFT_SELF_ANY=6.00
PRIMER_RIGHT_SELF_ANY=5.00
PRIMER_LEFT_SELF_END=4.00
PRIMER_RIGHT_SELF_END=0.00
PRIMER_LEFT_END_STABILITY=5.8000
PRIMER_RIGHT_END_STABILITY=7.9000
PRIMER_PAIR_MAX_COMPL_ANY=4.00
PRIMER_PAIR_MAX_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=147
PRIMER_PAIR_PENALTY_1=7.6879
PRIMER_LEFT_1_PENALTY=6.971746
PRIMER_RIGHT_1_PENALTY=0.716126
PRIMER_LEFT_1_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_1_SEQUENCE=CAGGGGCTGTTTCTGATTCT
PRIMER_LEFT_1=20,24
PRIMER_RIGHT_1=150,20
PRIMER_LEFT_1_TM=57.028
PRIMER_RIGHT_1_TM=59.284
PRIMER_LEFT_1_GC_PERCENT=33.333
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_LEFT_1_SELF_ANY=6.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=1.00
PRIMER_LEFT_1_END_STABILITY=5.8000
PRIMER_RIGHT_1_END_STABILITY=6.6000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=131
PRIMER_PAIR_PENALTY_2=7.8310
PRIMER_LEFT_2_PENALTY=6.971746
PRIMER_RIGHT_2_PENALTY=0.859212
PRIMER_LEFT_2_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_2_SEQUENCE=ACAGGGGCTGTTTCTGATTC
PRIMER_LEFT_2=20,24
PRIMER_RIGHT_2=151,20
PRIMER_LEFT_2_TM=57.028
PRIMER_RIGHT_2_TM=59.141
PRIMER_LEFT_2_GC_PERCENT=33.333
PRIMER_RIGHT_2_GC_PERCENT=50.000
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_LEFT_2_SELF_END=4.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=5.8000
PRIMER_RIGHT_2_END_STABILITY=6.6000
PRIMER_PAIR_2_COMPL_ANY=5.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=132
PRIMER_PAIR_PENALTY_3=7.8310
PRIMER_LEFT_3_PENALTY=6.971746
PRIMER_RIGHT_3_PENALTY=0.859212
PRIMER_LEFT_3_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_3_SEQUENCE=GAACAGGGGCTGTTTCTGAT
PRIMER_LEFT_3=20,24
PRIMER_RIGHT_3=153,20
PRIMER_LEFT_3_TM=57.028
PRIMER_RIGHT_3_TM=59.141
PRIMER_LEFT_3_GC_PERCENT=33.333
PRIMER_RIGHT_3_GC_PERCENT=50.000
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=7.00
PRIMER_LEFT_3_SELF_END=4.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_LEFT_3_END_STABILITY=5.8000
PRIMER_RIGHT_3_END_STABILITY=6.6000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_3=134
PRIMER_PAIR_PENALTY_4=8.0391
PRIMER_LEFT_4_PENALTY=6.971746
PRIMER_RIGHT_4_PENALTY=1.067353
PRIMER_LEFT_4_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_4_SEQUENCE=GGGCTGTTTCTGATTCTTTCC
PRIMER_LEFT_4=20,24
PRIMER_RIGHT_4=147,21
PRIMER_LEFT_4_TM=57.028
PRIMER_RIGHT_4_TM=60.067
PRIMER_LEFT_4_GC_PERCENT=33.333
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=6.00
PRIMER_RIGHT_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_LEFT_4_END_STABILITY=5.8000
PRIMER_RIGHT_4_END_STABILITY=8.5000
PRIMER_PAIR_4_COMPL_ANY=5.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_4=128
PRIMER_PAIR_PENALTY_5=8.0391
PRIMER_LEFT_5_PENALTY=6.971746
PRIMER_RIGHT_5_PENALTY=1.067353
PRIMER_LEFT_5_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_5_SEQUENCE=GGGGCTGTTTCTGATTCTTTC
PRIMER_LEFT_5=20,24
PRIMER_RIGHT_5=148,21
PRIMER_LEFT_5_TM=57.028
PRIMER_RIGHT_5_TM=60.067
PRIMER_LEFT_5_GC_PERCENT=33.333
PRIMER_RIGHT_5_GC_PERCENT=47.619
PRIMER_LEFT_5_SELF_ANY=6.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=4.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=5.8000
PRIMER_RIGHT_5_END_STABILITY=7.0000
PRIMER_PAIR_5_COMPL_ANY=5.00
PRIMER_PAIR_5_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_5=129
PRIMER_PAIR_PENALTY_6=8.0963
PRIMER_LEFT_6_PENALTY=6.971746
PRIMER_RIGHT_6_PENALTY=1.124598
PRIMER_LEFT_6_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_6_SEQUENCE=ACAGGGGCTGTTTCTGATTCT
PRIMER_LEFT_6=20,24
PRIMER_RIGHT_6=151,21
PRIMER_LEFT_6_TM=57.028
PRIMER_RIGHT_6_TM=60.125
PRIMER_LEFT_6_GC_PERCENT=33.333
PRIMER_RIGHT_6_GC_PERCENT=47.619
PRIMER_LEFT_6_SELF_ANY=6.00
PRIMER_RIGHT_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=4.00
PRIMER_RIGHT_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=5.8000
PRIMER_RIGHT_6_END_STABILITY=6.6000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=132
PRIMER_PAIR_PENALTY_7=8.1993
PRIMER_LEFT_7_PENALTY=6.971746
PRIMER_RIGHT_7_PENALTY=1.227592
PRIMER_LEFT_7_SEQUENCE=TGATCAGGATTTCAGTGCTAATAT
PRIMER_RIGHT_7_SEQUENCE=TTTGTGTTGCTTGCATTTTTG
PRIMER_LEFT_7=20,24
PRIMER_RIGHT_7=192,21
PRIMER_LEFT_7_TM=57.028
PRIMER_RIGHT_7_TM=59.772
PRIMER_LEFT_7_GC_PERCENT=33.333
PRIMER_RIGHT_7_GC_PERCENT=33.333
PRIMER_LEFT_7_SELF_ANY=6.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=4.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=5.8000
PRIMER_RIGHT_7_END_STABILITY=7.6000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=3.00
PRIMER_PRODUCT_SIZE_7=173
=
MARKER_NAME=10.RHAP34FA10.seq
SEQUENCE=NNNNNNNNNNNNACACATCTGAGAGTAGGGCAACCCCTGGGGTTCAAAACAGAAAGAAAGAATTCATTATTTAGGATAGAAAGGCAAGTAAATATAGATATCCTGCAATGGGATGAATACAAAGATTTATTTATTTATATTTATATTCATTAATCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCATCAGAGGACAGGTTGTAGGAGCCAGTTCAGTTCTGTCCTTGCATACCTACATTCTGGGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
INCLUDED_REGION=12,236
TARGET=155,32,TG
PRIMER_PAIR_PENALTY=1.7506
PRIMER_LEFT_PENALTY=0.649665
PRIMER_RIGHT_PENALTY=1.100969
PRIMER_LEFT_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_SEQUENCE=CAAGGACAGAACTGAACTGGC
PRIMER_LEFT=101,20
PRIMER_RIGHT=229,21
PRIMER_LEFT_TM=59.350
PRIMER_RIGHT_TM=59.899
PRIMER_LEFT_GC_PERCENT=45.000
PRIMER_RIGHT_GC_PERCENT=52.381
PRIMER_LEFT_SELF_ANY=4.00
PRIMER_RIGHT_SELF_ANY=4.00
PRIMER_LEFT_SELF_END=4.00
PRIMER_RIGHT_SELF_END=3.00
PRIMER_LEFT_END_STABILITY=5.6000
PRIMER_RIGHT_END_STABILITY=9.7000
PRIMER_PAIR_MAX_COMPL_ANY=3.00
PRIMER_PAIR_MAX_COMPL_END=0.00
PRIMER_PRODUCT_SIZE=129
PRIMER_PAIR_PENALTY_1=1.7506
PRIMER_LEFT_1_PENALTY=0.649665
PRIMER_RIGHT_1_PENALTY=1.100969
PRIMER_LEFT_1_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_1_SEQUENCE=GCAAGGACAGAACTGAACTGG
PRIMER_LEFT_1=101,20
PRIMER_RIGHT_1=230,21
PRIMER_LEFT_1_TM=59.350
PRIMER_RIGHT_1_TM=59.899
PRIMER_LEFT_1_GC_PERCENT=45.000
PRIMER_RIGHT_1_GC_PERCENT=52.381
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=4.00
PRIMER_RIGHT_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=5.6000
PRIMER_RIGHT_1_END_STABILITY=7.9000
PRIMER_PAIR_1_COMPL_ANY=3.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_1=130
PRIMER_PAIR_PENALTY_2=2.3642
PRIMER_LEFT_2_PENALTY=1.263200
PRIMER_RIGHT_2_PENALTY=1.100969
PRIMER_LEFT_2_SEQUENCE=GATATCCTGCAATGGGATGAA
PRIMER_RIGHT_2_SEQUENCE=CAAGGACAGAACTGAACTGGC
PRIMER_LEFT_2=96,21
PRIMER_RIGHT_2=229,21
PRIMER_LEFT_2_TM=59.737
PRIMER_RIGHT_2_TM=59.899
PRIMER_LEFT_2_GC_PERCENT=42.857
PRIMER_RIGHT_2_GC_PERCENT=52.381
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=6.9000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_2=134
PRIMER_PAIR_PENALTY_3=2.3642
PRIMER_LEFT_3_PENALTY=1.263200
PRIMER_RIGHT_3_PENALTY=1.100969
PRIMER_LEFT_3_SEQUENCE=GATATCCTGCAATGGGATGAA
PRIMER_RIGHT_3_SEQUENCE=GCAAGGACAGAACTGAACTGG
PRIMER_LEFT_3=96,21
PRIMER_RIGHT_3=230,21
PRIMER_LEFT_3_TM=59.737
PRIMER_RIGHT_3_TM=59.899
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=52.381
PRIMER_LEFT_3_SELF_ANY=6.00
PRIMER_RIGHT_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=6.9000
PRIMER_RIGHT_3_END_STABILITY=7.9000
PRIMER_PAIR_3_COMPL_ANY=4.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_3=135
PRIMER_PAIR_PENALTY_4=2.5784
PRIMER_LEFT_4_PENALTY=0.649665
PRIMER_RIGHT_4_PENALTY=1.928763
PRIMER_LEFT_4_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_4_SEQUENCE=TGCAAGGACAGAACTGAACTG
PRIMER_LEFT_4=101,20
PRIMER_RIGHT_4=231,21
PRIMER_LEFT_4_TM=59.350
PRIMER_RIGHT_4_TM=59.071
PRIMER_LEFT_4_GC_PERCENT=45.000
PRIMER_RIGHT_4_GC_PERCENT=47.619
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=4.00
PRIMER_RIGHT_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=5.6000
PRIMER_RIGHT_4_END_STABILITY=6.7000
PRIMER_PAIR_4_COMPL_ANY=4.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_4=131
PRIMER_PAIR_PENALTY_5=2.6739
PRIMER_LEFT_5_PENALTY=1.427458
PRIMER_RIGHT_5_PENALTY=1.246400
PRIMER_LEFT_5_SEQUENCE=TGGGGTTCAAAACAGAAAGAA
PRIMER_RIGHT_5_SEQUENCE=GGCTCCTACAACCTGTCCTCT
PRIMER_LEFT_5=37,21
PRIMER_RIGHT_5=211,21
PRIMER_LEFT_5_TM=59.573
PRIMER_RIGHT_5_TM=59.754
PRIMER_LEFT_5_GC_PERCENT=38.095
PRIMER_RIGHT_5_GC_PERCENT=57.143
PRIMER_LEFT_5_SELF_ANY=3.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_LEFT_5_SELF_END=0.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=7.0000
PRIMER_RIGHT_5_END_STABILITY=7.9000
PRIMER_PAIR_5_COMPL_ANY=4.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PRODUCT_SIZE_5=175
PRIMER_PAIR_PENALTY_6=2.6834
PRIMER_LEFT_6_PENALTY=1.427458
PRIMER_RIGHT_6_PENALTY=1.255934
PRIMER_LEFT_6_SEQUENCE=TGGGGTTCAAAACAGAAAGAA
PRIMER_RIGHT_6_SEQUENCE=GGCTCCTACAACCTGTCCTC
PRIMER_LEFT_6=37,21
PRIMER_RIGHT_6=211,20
PRIMER_LEFT_6_TM=59.573
PRIMER_RIGHT_6_TM=58.744
PRIMER_LEFT_6_GC_PERCENT=38.095
PRIMER_RIGHT_6_GC_PERCENT=60.000
PRIMER_LEFT_6_SELF_ANY=3.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=0.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=7.0000
PRIMER_RIGHT_6_END_STABILITY=7.9000
PRIMER_PAIR_6_COMPL_ANY=4.00
PRIMER_PAIR_6_COMPL_END=1.00
PRIMER_PRODUCT_SIZE_6=175
PRIMER_PAIR_PENALTY_7=2.7270
PRIMER_LEFT_7_PENALTY=0.649665
PRIMER_RIGHT_7_PENALTY=2.077340
PRIMER_LEFT_7_SEQUENCE=CCTGCAATGGGATGAATACA
PRIMER_RIGHT_7_SEQUENCE=AAGGACAGAACTGAACTGGCTC
PRIMER_LEFT_7=101,20
PRIMER_RIGHT_7=228,22
PRIMER_LEFT_7_TM=59.350
PRIMER_RIGHT_7_TM=59.923
PRIMER_LEFT_7_GC_PERCENT=45.000
PRIMER_RIGHT_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=4.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_LEFT_7_SELF_END=4.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_LEFT_7_END_STABILITY=5.6000
PRIMER_RIGHT_7_END_STABILITY=9.4000
PRIMER_PAIR_7_COMPL_ANY=3.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PRODUCT_SIZE_7=128
=
MARKER_NAME=13.RHAP34FB1.seq
SEQUENCE=NNNNNNNNNNNNNNNNNNNNNCNNNNNNNNNNNNNNNNGAGAGGTGTATATAGAGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTGTATCCAAAACTCTTTAGTCTTAATGTATTTATATACCTTTATTGTTATAAAATTCCTGTTTAAAACACAGTCTGAACAAGGTGACTTCTTTTAGAATTTGTCAGTAAGGAACACATTTTTTCTTTACCTATGTCTTTTTCTTAGACTGGGTCAGCACAATATTTGTAACTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
INCLUDED_REGION=21,244
TARGET=22,16,TA
TARGET=57,34,GT
=
MARKER_NAME=14.RHAP34FB2.seq
SEQUENCE=NNNNNNNNNNNNNACTCATATACATAAAATAAATAGATATTTTTAAATAAACAGAAGATTGCAACGAAATCAAGCAGAATTCAAGACAATGAATAAATAACAAAATCTAAAAGGCTCAAGACTGCAACACTCCTGTGGGAGCACACATGTGTGCATGGAACACNCAAATGCGCNCNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTNTNCACACAANNCTACNGCNNCACNNTTAGCACNCGCACAAGNNNNNNNNNNNNNNTTCNGCNACNACATNNNNANNNCNCNAANACNNCNACNNCNCNNTNNC
INCLUDED_REGION=13,303
TARGET=177,33,AC
TARGET=254,14,AC
=
